View primary antibody

Basic data

PIDprimary-0111
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0111
Research groupRG Enderlein
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBA4A
Antibody Registry ID(s)AB_477583
NameAnti-α-Tubulin antibody
Alternative nameH2-ALPHA
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts with bovine, rat, yeast, human, mouse, chicken, fungi, amphibia
CloneDM1A
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenMicrotubules from the brain of Chicken embryos
Crafted Bycompany
Company / ManufacturerSigma-Aldrich
Catalog no.T6199
Lot no.
Descriptionsfb1565: primary-0018
Localization
Storage instructionStore at 2-8 ° C for up to 1 month. Freeze for long-term storage in working aliquots at -20 ° C. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If the solution has become slightly cloudy after long periods of storage, clarify the solution by centrifugation before use. Unused working thinners should be discarded after 12 hours.
Receipt date2021-01-01 00:00:00
Preparation date2021-01-29 00:00:00
Created byKlauenberg, Vanessa
Last modified,

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Linked publications


Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
Thiele JC, Helmerich DA, Oleksiievets N, Tsukanov R, Butkevich E, Sauer M, Nevskyi O, Enderlein J
ACS Nano 2020 : 10.1021/acsnano.0c07322

Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy
Qin S, Isbaner S, Gregor I, Enderlein J
Nat Protoc 2020 : 10.1038/s41596-020-00408-x

The RNA methyltransferase METTL8 installs m3C32 in mitochondrial tRNAsThr/Ser(UCN) to optimise tRNA structure and mitochondrial translation
Kleiber N, Lemus-Diaz N, Stiller C, Heinrichs M, Mai MM, Hackert P, Richter-Dennerlein R, Höbartner C, Bohnsack KE, Bohnsack MT
Nat Commun 2022 : 10.1038/s41467-021-27905-1

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds
Saha I, Yuste-Checa P, Silva Padilha MD, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Ulrich Hartl F, Hipp MS
bioRxiv 2022 : 10.1101/2022.02.18.481043

Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schöler HR
eLife 2022 : 10.7554/elife.71533

Aromaticity at position 39 in α‐synuclein: A modulator of amyloid fibril assembly and membrane‐bound conformations
Buratti FA, Boeffinger N, Garro HA, Flores JS, Hita FJ, Gonçalves PdC, Copello FdR, Lizarraga L, Rossetti G, Carloni P, Zweckstetter M, Outeiro TF, Eimer S, Griesinger C, Fernández CO
Protein Sci 2022 : 10.1002/pro.4360

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS
Nat Commun 2023 : 10.1038/s41467-023-36058-2

Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices
Jentoft IM, Bäuerlein FJ, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M
Cell 2023 : 10.1016/j.cell.2023.10.003

Autophagy preferentially degrades non-fibrillar polyQ aggregates
Zhao DY, Bäuerlein FJ, Saha I, Hartl FU, Baumeister W, Wilfling F
Mol Cell 2024 : 10.1016/j.molcel.2024.04.018

One-step nanoscale expansion microscopy reveals individual protein shapes
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO
Nat Biotechnol 2024 : 10.1038/s41587-024-02431-9