| Property |
Value |
| Working Groups |
RG Fernandez-Busnadiego |
| Subproject |
RA2.1 |
| Open Access |
Yes |
| Publication Type |
Journal Article |
| Peer Reviewed |
Yes |
| PMID |
36732333 |
| DOI |
10.1038/s41467-023-36058-2 |
| Publication Year |
2023 |
| Title |
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system |
| Journal |
Nature Communications |
| ISSN |
2041-1723 |
| eISSN |
2041-1723 |
| URL |
http://dx.doi.org/10.1038/s41467-023-36058-2 |
| Issue |
1 |
| Volume |
14 |
| Journal Abbreviation |
Nat Commun |
| Authors |
Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS |
| First Author |
Saha I |
| Last Author |
Hipp MS |
External Resources
12259https://www.addgene.org/12259 Addgene identifier (12259, reused)
12260https://www.addgene.org/12260 Addgene identifier (12260, reused)
23971https://www.addgene.org/23971 Addgene identifier (23971, reused)
23972https://www.addgene.org/23972 Addgene identifier (23972, reused)
23973https://www.addgene.org/23973 Addgene identifier (23973, reused)
23974https://www.addgene.org/23974 Addgene identifier (23974, reused)
100521https://www.addgene.org/100521 Addgene identifier (100521, reused)
188572https://www.addgene.org/188572 Addgene identifier (188572)
188573https://www.addgene.org/188573 Addgene identifier (188573)
nature.c...-2.pdfhttps://www.nature.com/articles/s41467-023-36058-2.pdf Article fulltext
EMD-13739https://www.ebi.ac.uk/pdbe/emdb/EMD-13739 EMBL-EBI EMDB identifier (EMD-13739)
EMD-13740https://www.ebi.ac.uk/pdbe/emdb/EMD-13740 EMBL-EBI EMDB identifier (EMD-13740)
13579https://instruments.goettingen-research-online.de/Landing/Resource?id=13579 GRO.instruments resource identifier (13579, Krios G4 Cryo-TEM)
2/121729http://resolver.sub.uni-goettingen.de/purl?2/121729 GRO.publications identifier
9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 NCBI taxonomy (human, Homo sapiens)
0000-0002-1056-3849https://orcid.org/0000-0002-1056-3849 ORCID identifier (Patricia Yuste Checa)
0000-0003-3520-5439https://orcid.org/0000-0003-3520-5439 ORCID identifier (Qiang Guo)
0000-0002-2050-413Xhttps://orcid.org/0000-0002-2050-413X ORCID identifier (Hauke Holthusen)
0000-0001-8964-6120https://orcid.org/0000-0001-8964-6120 ORCID identifier (Victoria Anne Trinkaus)
0000-0003-1052-8485https://orcid.org/0000-0003-1052-8485 ORCID identifier (Irina Dudanova)
0000-0002-8366-7622https://orcid.org/0000-0002-8366-7622 ORCID identifier (Ruben Fernandez-Busnadiego)
0000-0001-8154-8809https://orcid.org/0000-0001-8154-8809 ORCID identifier (Wolfgang Baumeister)
0000-0003-0366-6169https://orcid.org/0000-0003-0366-6169 ORCID identifier (Saurabh Gautam)
0000-0002-8085-7770https://orcid.org/0000-0002-8085-7770 ORCID identifier (Marc I. Diamond)
0000-0002-7941-135Xhttps://orcid.org/0000-0002-7941-135X ORCID identifier (F. Ulrich Hartl)
PXD023400http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD023400 Pride proteome dataset (PXD023400)
449https://prod.mbexc.uni-goettingen.de/literature/publications/449 Published Data Registry entry (MBExC, preprint)
1/122https://mbexc.uni-goettingen.de/archive/1/122 Research Data Archive (supplemental material)
05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)
021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)
AB_223647https://scicrunch.org/resolver/AB_223647 SciCrunch identifier (RRID:AB_223647)
AB_258167https://scicrunch.org/resolver/AB_258167 SciCrunch identifier (RRID:AB_258167)
AB_258434https://scicrunch.org/resolver/AB_258434 SciCrunch identifier (RRID:AB_258434)
AB_298039https://scicrunch.org/resolver/AB_298039 SciCrunch identifier (RRID:AB_298039)
AB_390913https://scicrunch.org/resolver/AB_390913 SciCrunch identifier (RRID:AB_390913)
AB_477583https://scicrunch.org/resolver/AB_477583 SciCrunch identifier (RRID:AB_477583)
AB_527461https://scicrunch.org/resolver/AB_527461 SciCrunch identifier (RRID:AB_527461)
AB_612093https://scicrunch.org/resolver/AB_612093 SciCrunch identifier (RRID:AB_612093)
AB_621843https://scicrunch.org/resolver/AB_621843 SciCrunch identifier (RRID:AB_621843)
AB_628423https://scicrunch.org/resolver/AB_628423 SciCrunch identifier (RRID:AB_628423)
AB_796155https://scicrunch.org/resolver/AB_796155 SciCrunch identifier (RRID:AB_796155)
AB_1587578https://scicrunch.org/resolver/AB_1587578 SciCrunch identifier (RRID:AB_1587578)
AB_1587580https://scicrunch.org/resolver/AB_1587580 SciCrunch identifier (RRID:AB_1587580)
AB_1854586https://scicrunch.org/resolver/AB_1854586 SciCrunch identifier (RRID:AB_1854586)
AB_2062340https://scicrunch.org/resolver/AB_2062340 SciCrunch identifier (RRID:AB_2062340)
AB_2107445https://scicrunch.org/resolver/AB_2107445 SciCrunch identifier (RRID:AB_2107445)
AB_2266850https://scicrunch.org/resolver/AB_2266850 SciCrunch identifier (RRID:AB_2266850)
AB_2268979https://scicrunch.org/resolver/AB_2268979 SciCrunch identifier (RRID:AB_2268979)
AB_2340863https://scicrunch.org/resolver/AB_2340863 SciCrunch identifier (RRID:AB_2340863)
AB_2341273https://scicrunch.org/resolver/AB_2341273 SciCrunch identifier (RRID:AB_2341273)
AB_2534032https://scicrunch.org/resolver/AB_2534032 SciCrunch identifier (RRID:AB_2534032)
AB_2534033https://scicrunch.org/resolver/AB_2534033 SciCrunch identifier (RRID:AB_2534033)
AB_2556746https://scicrunch.org/resolver/AB_2556746 SciCrunch identifier (RRID:AB_2556746)
AB_10678868https://scicrunch.org/resolver/AB_10678868 SciCrunch identifier (RRID:AB_10678868)
AB_10831194https://scicrunch.org/resolver/AB_10831194 SciCrunch identifier (RRID:AB_10831194)
AB_10956588https://scicrunch.org/resolver/AB_10956588 SciCrunch identifier (RRID:AB_10956588)
AB_10980631https://scicrunch.org/resolver/AB_10980631 SciCrunch identifier (RRID:AB_10980631)
AB_11167058https://scicrunch.org/resolver/AB_11167058 SciCrunch identifier (RRID:AB_11167058)
CVCL_0045https://scicrunch.org/resolver/CVCL_0045 SciCrunch identifier (RRID:CVCL_0045, HEK293)
CVCL_0063https://scicrunch.org/resolver/CVCL_0063 SciCrunch identifier (RRID:CVCL_0063, HEK293T)
CVCL_C1CRhttps://scicrunch.org/resolver/CVCL_C1CR SciCrunch identifier (RRID:CVCL_C1CR, HEK293T Tet-off FLTau)
CVCL_C1CShttps://scicrunch.org/resolver/CVCL_C1CS SciCrunch identifier (RRID:CVCL_C1CS, HEK293T Tet-off TauRD)
SCR_007353https://scicrunch.org/resolver/SCR_007353 SciCrunch identifier (RRID:SCR_007353, Advanced 3D Visualization and Volume Modeling)
SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)
SCR_008520https://scicrunch.org/resolver/SCR_008520 SciCrunch identifier (RRID:SCR_008520, FlowJo)
SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)
SCR_003297https://scicrunch.org/resolver/SCR_003297 SciCrunch identifier (RRID:SCR_003297, IMOD)
SCR_014485https://scicrunch.org/resolver/SCR_014485 SciCrunch identifier (RRID:SCR_014485, MaxQuant)
SCR_016137https://scicrunch.org/resolver/SCR_016137 SciCrunch identifier (RRID:SCR_016137, Microsoft Excel)
SCR_016499https://scicrunch.org/resolver/SCR_016499 SciCrunch identifier (RRID:SCR_016499, MotionCor2)
SCR_014212https://scicrunch.org/resolver/SCR_014212 SciCrunch identifier (RRID:SCR_014212, Origin)
SCR_015753https://scicrunch.org/resolver/SCR_015753 SciCrunch identifier (RRID:SCR_015753, Perseus)
SCR_017293https://scicrunch.org/resolver/SCR_017293 SciCrunch identifier (RRID:SCR_017293, SerialEM)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM1_ESM.pdf Supplemental material
goeseek.gwdguser.de/studies/51https://goeseek.gwdguser.de/studies/51 Supplemental material (goeseek study)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM2_ESM.pdf Supplemental material (peer review)
dx.doi.o...gmk/v1https://dx.doi.org/10.17504/protocols.io.6qpvr4xn3gmk/v1 Supplemental material (protocols.io)
dx.doi.o...g25/v1https://dx.doi.org/10.17504/protocols.io.kqdg39eo1g25/v1 Supplemental material (protocols.io)
dx.doi.o...lr2/v1https://dx.doi.org/10.17504/protocols.io.ewov1ojwklr2/v1 Supplemental material (protocols.io)
dx.doi.o...g3e/v1https://dx.doi.org/10.17504/protocols.io.x54v9den4g3e/v1 Supplemental material (protocols.io)
dx.doi.o...l5b/v1https://dx.doi.org/10.17504/protocols.io.n92ldpbx8l5b/v1 Supplemental material (protocols.io)
dx.doi.o...lo5/v1https://dx.doi.org/10.17504/protocols.io.3byl4je4rlo5/v1 Supplemental material (protocols.io)
dx.doi.o...vk5/v1https://dx.doi.org/10.17504/protocols.io.36wgqjrzyvk5/v1 Supplemental material (protocols.io)
dx.doi.o...g3e/v1https://dx.doi.org/10.17504/protocols.io.x54v9jnrzg3e/v1 Supplemental material (protocols.io)
dx.doi.o...g1y/v1https://dx.doi.org/10.17504/protocols.io.4r3l27mjpg1y/v1 Supplemental material (protocols.io)
static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM3_ESM.pdf Supplemental material (reporting summary)
static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM4_ESM.xlsx Supplemental material (source data)
UP000005640https://www.uniprot.org/proteomes/UP000005640 UniProt Proteome identifier (UP000005640)
mbexc.de...ystem/https://mbexc.de/the-aaa-chaperone-vcp-disaggregates-tau-fibrils-and-generates-aggregate-seeds-in-a-cellular-system/ Website entry (mbexc.de)
Linked Antibodies
| PID |
AG |
Type |
Antigen symbol |
Antibody Registry ID |
Name |
| primary-0008 |
 |
primary |
GAPDH |
AB_2107445 |
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody |
| primary-0111 |
 |
primary |
TUBA4A |
AB_477583 |
Anti-α-Tubulin antibody |
| primary-0185 |
 |
primary |
MAPT |
AB_223647 |
Phospho-Tau (Ser202, Thr205) Monoclonal Antibody |
| primary-0190 |
|
primary |
UBB |
AB_1587578 |
Anti-Ubiquitin Antibody |
| primary-0191 |
|
primary |
UBB |
AB_1587580 |
Anti-Ubiquitin Antibody |
| primary-0193 |
|
primary |
VCP |
AB_298039 |
Anti-VCP antibody |
| primary-0619 |
 |
primary |
ATG7 |
AB_10831194 |
Atg7 (D12B11) antibody |
| primary-0784 |
 |
primary |
GFP |
AB_390913 |
Anti-GFP antibody |
| primary-0990 |
|
primary |
UBB |
AB_628423 |
Anti-Ubiquitin Antibody (P4D1) |
| primary-0991 |
|
primary |
MAPT |
AB_11167058 |
Tau (phospho Ser356) antibody |
| primary-0992 |
|
primary |
NPLOC4 |
AB_1854586 |
Anti-NPLOC4 antibody |
| primary-0993 |
|
primary |
UFD1 |
AB_10678868 |
Anti-UFD1L antibody |
| primary-0994 |
|
primary |
Ubiquitinylated proteins |
AB_612093 |
Anti-Ubiquitinylated proteins Antibody |
| primary-0995 |
|
primary |
MAPT |
AB_10980631 |
Tau Monoclonal Antibody |
| primary-0996 |
|
primary |
MAPT |
AB_2341273 |
Anti-Human Tau/Repeat Domain (2B11) Mouse IgG MoAb |
| primary-0997 |
|
primary |
MAP1LC3B |
AB_796155 |
Anti-LC3B antibody |
| primary-0998 |
|
primary |
ATG5 |
AB_2062340 |
Atg5 Antibody |
| primary-0999 |
|
primary |
PSMD11 |
AB_2268979 |
PSMD11 Polyclonal antibody |
| primary-1199 |
|
primary |
VCP |
AB_527461 |
p97/VCP Antibody |
| primary-1200 |
|
primary |
MYC |
AB_2266850 |
c-Myc antibody (9E 10) |
| secondary-0042 |
 |
secondary |
|
AB_2534033 |
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 |
| secondary-0376 |
|
secondary |
|
AB_258434 |
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody |
| secondary-1000 |
|
secondary |
|
AB_2534032 |
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5 |
| secondary-1001 |
|
secondary |
|
AB_2340863 |
Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L) |
| secondary-1002 |
|
secondary |
|
AB_2556746 |
Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488 |
| secondary-1003 |
|
secondary |
|
AB_258167 |
Anti-Mouse IgG (whole molecule)–Peroxidase antibody |
| secondary-1004 |
|
secondary |
|
AB_10956588 |
IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody |
| secondary-1005 |
|
secondary |
|
AB_621843 |
IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody |