Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0990 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0990 |
| Research group | RG Fernandez-Busnadiego |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | UBB |
| Antibody Registry ID(s) | AB_628423 |
| Name | Anti-Ubiquitin Antibody (P4D1) |
| Alternative name | polyubiquitin B |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | Mouse, Rat, Human, Drosophila |
| Clone | P4D1 |
| Isotype | IgG1κ |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | raised against amino acids 1-76 representing full length Ubiquitin of bovine origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-8017 |
| Lot no. | |
| Description | umg-sfb1190-antibody-primary-0879 Western Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 μg per 1 x 106 cells) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required |
| Receipt date | 2022-01-01 00:00:00 |
| Preparation date | 2022-03-18 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=UBB |
| Wikipedia | http://en.wikipedia.org/wiki/UBB |
| Uniprot | http://www.uniprot.org/uniprot/?query=UBB |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=UBB |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_628423 |
Applications
No application comments yet.
Linked publications
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds
Saha I, Yuste-Checa P, Silva Padilha MD, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Ulrich Hartl F, Hipp MS
bioRxiv 2022 : 10.1101/2022.02.18.481043
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS
Nat Commun 2023 : 10.1038/s41467-023-36058-2
Nedd4-2-dependent regulation of astrocytic Kir4.1 and Connexin43 controls neuronal network activity
Altas B, Rhee H, Ju A, Solís HC, Karaca S, Winchenbach J, Kaplan-Arabaci O, Schwark M, Ambrozkiewicz MC, Lee C, Spieth L, Wieser GL, Chaugule VK, Majoul I, Hassan MA, Goel R, Wojcik SM, Koganezawa N, Hanamura K, Rotin D, Pichler A, Mitkovski M, de Hoz L, Poulopoulos A, Urlaub H, Jahn O, Saher G, Brose N, Rhee J, Kawabe H
J Cell Biol 2023 : 10.1083/jcb.201902050
