View primary antibody

Basic data

PIDprimary-0681
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0681
Research groupRG Gollisch
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolHTT
Antibody Registry ID(s)AB_177645
NameAnti-Huntingtin Protein Antibody
Alternative nameSLC6A4
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withHuman, Rat, Mouse
ClonemEM48
IsotypeIgG
Clonalitymonoclonal
Demaskingunknown
AntigenGST fusion protein from the first 256 amino acids from human huntingtin with the deletion of the polyglutamine tract.
Crafted Bycompany
Company / ManufacturerMerck Millipore
Catalog no.MAB5374
Lot no.
DescriptionImmunohistochemistry: 1:50-1:100 of a previous lot using ABC on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Yu, Z et al (2002) Hum. Mole. Genetics 11(8):905-914. (http://hmg.oxfordjournals.org/cgi/content/full/11/8/905) for good IHC methods and photos of mEM48 on rodent tissues with human transgenic material. Immunocytochemistry: light 4% PFA fixation followed by 0.1% triton X-100 incubation prior to blocking is suggested. A previous lot of this antibody was used in IC. Western blot: 1:50-1:500 using ECL depending on the level of mutant protein. Suggested dilution buffer is PBS containing 3% BSA or PBS containing 5% non-fat milk. Nuclear fraction preparations enhance signals; monomeric protein ~80kDa; aggregates are common which can be > 200kDa in size. Optimal working dilutions must be determined by the end user.
Localization
Storage instructionStable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Receipt date2021-08-31 00:00:00
Preparation date2021-08-31 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825