| Antigen symbol |
HTT |
| Antibody Registry ID(s) |
AB_177645 |
| Name |
Anti-Huntingtin Protein Antibody |
| Alternative name |
SLC6A4 |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
human, rat, mouse |
| Clone |
mEM48 |
| Isotype |
IgG |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
GST fusion protein from the first 256 amino acids from human huntingtin with the deletion of the polyglutamine tract. |
| Crafted By |
company |
| Company / Manufacturer |
Merck Millipore |
| Catalog no. |
MAB5374 |
| Lot no. |
|
| Description |
Immunohistochemistry:
1:50-1:100 of a previous lot using ABC on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections.
Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Yu, Z et al (2002) Hum. Mole. Genetics 11(8):905-914. (http://hmg.oxfordjournals.org/cgi/content/full/11/8/905) for good IHC methods and photos of mEM48 on rodent tissues with human transgenic material.
Immunocytochemistry:
light 4% PFA fixation followed by 0.1% triton X-100 incubation prior to blocking is suggested.
A previous lot of this antibody was used in IC.
Western blot:
1:50-1:500 using ECL depending on the level of mutant protein. Suggested dilution buffer is PBS containing 3% BSA or PBS containing 5% non-fat milk.
Nuclear fraction preparations enhance signals; monomeric protein ~80kDa; aggregates are common which can be > 200kDa in size.
Optimal working dilutions must be determined by the end user. |
| Localization |
|
| Storage instruction |
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Receipt date |
2021-08-31 00:00:00 |
| Preparation date |
2021-08-31 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |