Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0681 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0681 |
| Research group | RG Gollisch |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | HTT |
| Antibody Registry ID(s) | AB_177645 |
| Name | Anti-Huntingtin Protein Antibody |
| Alternative name | SLC6A4 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | human, rat, mouse |
| Clone | mEM48 |
| Isotype | IgG |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | GST fusion protein from the first 256 amino acids from human huntingtin with the deletion of the polyglutamine tract. |
| Crafted By | company |
| Company / Manufacturer | Merck Millipore |
| Catalog no. | MAB5374 |
| Lot no. | |
| Description | Immunohistochemistry: 1:50-1:100 of a previous lot using ABC on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Yu, Z et al (2002) Hum. Mole. Genetics 11(8):905-914. (http://hmg.oxfordjournals.org/cgi/content/full/11/8/905) for good IHC methods and photos of mEM48 on rodent tissues with human transgenic material. Immunocytochemistry: light 4% PFA fixation followed by 0.1% triton X-100 incubation prior to blocking is suggested. A previous lot of this antibody was used in IC. Western blot: 1:50-1:500 using ECL depending on the level of mutant protein. Suggested dilution buffer is PBS containing 3% BSA or PBS containing 5% non-fat milk. Nuclear fraction preparations enhance signals; monomeric protein ~80kDa; aggregates are common which can be > 200kDa in size. Optimal working dilutions must be determined by the end user. |
| Localization | |
| Storage instruction | Stable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Receipt date | 2021-08-31 00:00:00 |
| Preparation date | 2021-08-31 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
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Linked publications
Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825
