[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"681","pid":"primary-0681","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0681","research_group":"RG Gollisch","lab_id":"","type":"primary","antigen_symbol":"HTT","antibody_registry_id":"AB_177645","name":"Anti-Huntingtin Protein Antibody","alternative_name":"SLC6A4","tag_flurophore":"","raised_id":"Mouse","reacts_with":"human, rat, mouse","clone":"mEM48","isotype":"IgG","clonality":"monoclonal","demasking":"unknown","antigen":"GST fusion protein from the first 256 amino acids from human huntingtin with the deletion of the polyglutamine tract.","company":"Merck Millipore","catalog_no":"MAB5374","lot_no":"","description":"Immunohistochemistry:\r\n1:50-1:100 of a previous lot using ABC on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections.\r\nSuggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Yu, Z et al (2002) Hum. Mole. Genetics 11(8):905-914. (http:\/\/hmg.oxfordjournals.org\/cgi\/content\/full\/11\/8\/905) for good IHC methods and photos of mEM48 on rodent tissues with human transgenic material.\r\n\r\nImmunocytochemistry:\r\nlight 4% PFA fixation followed by 0.1% triton X-100 incubation prior to blocking is suggested.\r\nA previous lot of this antibody was used in IC.\r\n\r\nWestern blot:\r\n1:50-1:500 using ECL depending on the level of mutant protein. Suggested dilution buffer is PBS containing 3% BSA or PBS containing 5% non-fat milk.\r\nNuclear fraction preparations enhance signals; monomeric protein ~80kDa; aggregates are common which can be \u0026gt; 200kDa in size.\r\n\r\nOptimal working dilutions must be determined by the end user.","last_modified":"Klauenberg, Vanessa"}]