View secondary antibody

Basic data

PID secondary-0376
EPIC PID
Research group RG Fernandez-Busnadiego
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antibody Registry ID(s) AB_258434
Name Anti-Rabbit IgG (whole molecule)–Peroxidase antibody
Alternative name
Lab ID
Tag / Fluorophore peroxidase conjugate
Raised in
Clone
Isotype IgG
Clonality
Antigen purified rabbit IgG
Excitation Max.
Emission Max.
Crafted By company
Company / Manufacturer Sigma-Aldrich
Catalog no. A9169
Lot no.
Description direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)
Localization
Storage instruction For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Receipt date 2021-04-20 00:00:00
Preparation date 2021-04-20 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

External links

Antibodypedia -
HGNC -
Wikipedia -
Uniprot -
GeneCards -
Antibody Registry https://antibodyregistry.org/search?q=AB_258434