Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View secondary antibody
Basic data
| PID | secondary-0376 |
| EPIC PID | https://hdl.handle.net/21.11124/secondary-0376 |
| Research group | RG Fernandez-Busnadiego |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antibody Registry ID(s) | AB_258434 |
| Name | Anti-Rabbit IgG (whole molecule)–Peroxidase antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | peroxidase conjugate |
| Raised in | Goat |
| Clone | |
| Isotype | IgG |
| Clonality | |
| Antigen | purified rabbit IgG |
| Excitation Max. | |
| Emission Max. | |
| Crafted By | company |
| Company / Manufacturer | Sigma-Aldrich |
| Catalog no. | A9169 |
| Lot no. | |
| Description | direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well) |
| Localization | |
| Storage instruction | For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Receipt date | 2021-04-20 00:00:00 |
| Preparation date | 2021-04-20 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | - |
| Wikipedia | - |
| Uniprot | - |
| GeneCards | - |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_258434 |
Applications
No application comments yet.
Linked publications
In situ architecture of neuronal α-Synuclein inclusions
Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJ, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R
bioRxiv 2020 : 10.1101/2020.08.07.234138
In situ architecture of neuronal α-Synuclein inclusions
Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJB, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R
Nat Commun 2021 : 10.1038/s41467-021-22108-0
Mechanism of spindle pole organization and instability in human oocytes
So C, Menelaou K, Uraji J, Harasimov K, Steyer AM, Seres KB, Bucevičius J, Lukinavičius G, Möbius W, Sibold C, Tandler-Schneider A, Eckel H, Moltrecht R, Blayney M, Elder K, Schuh M
Science 2022 : 10.1126/science.abj3944
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds
Saha I, Yuste-Checa P, Silva Padilha MD, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Ulrich Hartl F, Hipp MS
bioRxiv 2022 : 10.1101/2022.02.18.481043
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS
Nat Commun 2023 : 10.1038/s41467-023-36058-2
Autophagy preferentially degrades non-fibrillar polyQ aggregates
Zhao DY, Bäuerlein FJ, Saha I, Hartl FU, Baumeister W, Wilfling F
Mol Cell 2024 : 10.1016/j.molcel.2024.04.018
