Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0984 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0984 |
| Research group | RG Möbius |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | ACTB |
| Antibody Registry ID(s) | AB_306371 |
| Name | Anti-beta Actin antibody [mAbcam 8226] - Loading Control |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | Mouse, Rat, Human |
| Clone | mAbcam 8226 |
| Isotype | IgG1 |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab8226 |
| Lot no. | |
| Description | ICC/IF Use a concentration of 5 µg/ml. IHC-P Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.Can be blocked with Human beta Actin peptide (ab13772). We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data. |
| Localization | Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2022-03-16 00:00:00 |
| Preparation date | 2022-03-16 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=ACTB |
| Wikipedia | http://en.wikipedia.org/wiki/ACTB |
| Uniprot | http://www.uniprot.org/uniprot/?query=ACTB |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=ACTB |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_306371 |
Applications
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Linked publications
Mechanism of spindle pole organization and instability in human oocytes
So C, Menelaou K, Uraji J, Harasimov K, Steyer AM, Seres KB, Bucevičius J, Lukinavičius G, Möbius W, Sibold C, Tandler-Schneider A, Eckel H, Moltrecht R, Blayney M, Elder K, Schuh M
Science 2022 : 10.1126/science.abj3944
