View primary antibody

Basic data

Research groupRG Möbius
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolACTB
Antibody Registry ID(s)AB_306371
NameAnti-beta Actin antibody [mAbcam 8226] - Loading Control
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human
ClonemAbcam 8226
AntigenSynthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab8226
Lot no.
DescriptionICC/IF Use a concentration of 5 µg/ml. IHC-P Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.Can be blocked with Human beta Actin peptide (ab13772). We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.
LocalizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2022-03-16 00:00:00
Preparation date2022-03-16 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications

Mechanism of spindle pole organization and instability in human oocytes
So C, Menelaou K, Uraji J, Harasimov K, Steyer AM, Seres KB, Bucevičius J, Lukinavičius G, Möbius W, Sibold C, Tandler-Schneider A, Eckel H, Moltrecht R, Blayney M, Elder K, Schuh M
Science 2022 : 10.1126/science.abj3944