| Antigen symbol |
ACTB |
| Antibody Registry ID(s) |
AB_306371 |
| Name |
Anti-beta Actin antibody [mAbcam 8226] - Loading Control |
| Alternative name |
|
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, human |
| Clone |
mAbcam 8226 |
| Isotype |
IgG1 |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab8226 |
| Lot no. |
|
| Description |
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.Can be blocked with Human beta Actin peptide (ab13772).
We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data. |
| Localization |
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2022-03-16 00:00:00 |
| Preparation date |
2022-03-16 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |