[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"984","pid":"primary-0984","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0984","research_group":"RG M\u00f6bius","lab_id":"","type":"primary","antigen_symbol":"ACTB ","antibody_registry_id":"AB_306371","name":"Anti-beta Actin antibody [mAbcam 8226] - Loading Control ","alternative_name":"","tag_flurophore":"","raised_id":"Mouse","reacts_with":"mouse, rat, human","clone":"mAbcam 8226","isotype":"IgG1","clonality":"monoclonal","demasking":"unknown","antigen":"Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin ","company":"Abcam","catalog_no":"ab8226","lot_no":"","description":"ICC\/IF \t \t\r\nUse a concentration of 5 \u00b5g\/ml.\r\n\r\nIHC-P \t\r\nUse a concentration of 0.1 - 0.5 \u00b5g\/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.\r\n\r\nWB \t\r\nUse a concentration of 1 \u00b5g\/ml. Predicted molecular weight: 42 kDa.Can be blocked with Human beta Actin peptide (ab13772).\r\n\r\nWe recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.","last_modified":"Klauenberg, Vanessa"}]