Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0848 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0848 |
| Research group | RG Fischer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | RBFOX3 |
| Antibody Registry ID(s) | AB_2532109 |
| Name | Recombinant Anti-NeuN antibody [EPR12763] - Neuronal Marker |
| Alternative name | FOX-3 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, CommonMarmoset |
| Clone | EPR12763 |
| Isotype | IgG |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab177487 |
| Lot no. | |
| Description | Flow Cyt (Intra) 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. IHC (PFA fixed) Use at an assay dependent concentration. mIHC 1/1000. Perform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. IHC-P 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/800. WB 1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/2000. ICC/IF 1/100 - 1/300. For unpurified use at 1/80. IHC-Fr Use at an assay dependent concentration. |
| Localization | Nucleus. Cytoplasm. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Receipt date | 2021-11-20 00:00:00 |
| Preparation date | 2021-11-20 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=RBFOX3 |
| Wikipedia | http://en.wikipedia.org/wiki/RBFOX3 |
| Uniprot | http://www.uniprot.org/uniprot/?query=RBFOX3 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=RBFOX3 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2532109 |
Applications
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Linked publications
Interferon-driven brain phenotype in a mouse model of RNaseT2 deficient leukoencephalopathy
Kettwig M, Ternka K, Wendland K, Krüger DM, Zampar S, Schob C, Franz J, Aich A, Winkler A, Sakib MS, Kaurani L, Epple R, Werner HB, Hakroush S, Kitz J, Prinz M, Bartok E, Hartmann G, Schröder S, Rehling P, Henneke M, Boretius S, Alia A, Wirths O, Fischer A, Stadelmann C, Nessler S, Gärtner J
Nat Commun 2021 : 10.1038/s41467-021-26880-x
