Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0711 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0711 |
| Research group | RG Rizzoli |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | DRD2 |
| Antibody Registry ID(s) | AB_2094980 |
| Name | Anti-Dopamine D2 Receptor Antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Human, Mouse, Rat, Monkey |
| Clone | |
| Isotype | |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic loop #3. |
| Crafted By | company |
| Company / Manufacturer | Merck Millipore |
| Catalog no. | AB5084P |
| Lot no. | |
| Description | Western blot: 1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands. Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections on a previous lot. Immunohistochemistry: 1-20 µg/mL on paraformaldehyde fixed sections. Immunoprecipitation: 1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2). ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA. Optimal working dilutions must be determined by the end user. |
| Localization | |
| Storage instruction | Stable for 1 year at -20ºC from date of receipt. |
| Receipt date | 2021-09-14 00:00:00 |
| Preparation date | 2021-09-14 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
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Linked publications
A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines
Helm MS, Dankovich TM, Mandad S, Rammner B, Jähne S, Salimi V, Koerbs C, Leibrandt R, Urlaub H, Schikorski T, Rizzoli SO
Nat Neurosci 2021 : 10.1038/s41593-021-00874-w
