[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"711","pid":"primary-0711","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0711","research_group":"RG Rizzoli","lab_id":"","type":"primary","antigen_symbol":"DRD2","antibody_registry_id":"AB_2094980","name":"Anti-Dopamine D2 Receptor Antibody","alternative_name":"","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"human, mouse, rat, monkey","clone":"","isotype":"","clonality":"polyclonal","demasking":"unknown","antigen":"A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic loop #3.","company":"Merck Millipore","catalog_no":"AB5084P ","lot_no":"","description":"Western blot:\r\n1-10 \u00b5g\/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui \u0026amp; Prasad 1992, BBRC; Brock et al. 1992; Farooqui \u0026amp; Prasad 1992, Life Sci). Approximately 100-400 \u00b5g of membrane protein\/lane should be loaded to visualize various bands.\r\n\r\nImmunohistochemistry:\r\n2-10 \u03bcg\/mL on paraformaldehyde fixed sections on a previous lot.\r\nImmunohistochemistry:\r\n1-20 \u00b5g\/mL on paraformaldehyde fixed sections.\r\n\r\nImmunoprecipitation:\r\n1-10 \u03bcg\/mL using 100-400 \u03bcg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).\r\n\r\nELISA:\r\n0.5-1.0 \u03bcg\/mL (1 \u03bcg\/mL immunogen peptide (Catalog number AG221)\/well) of a previous lot was used on ELISA.\r\n\r\nOptimal working dilutions must be determined by the end user.","last_modified":"Klauenberg, Vanessa"}]