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Publication

Property	Value
Working Groups	RG Rizzoli, RG Urlaub
Subproject	RA2.2, RA3.1, RA3.2
Open Access	No
Publication Type	Journal Article
Peer Reviewed	Yes
PMID	34168338
DOI	10.1038/s41593-021-00874-w
Publication Year	2021
Title	A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines
Journal	Nature Neuroscience
ISSN	1097-6256
eISSN	1546-1726
URL	http://dx.doi.org/10.1038/s41593-021-00874-w
Journal Abbreviation	Nat Neurosci
Authors	Helm MS, Dankovich TM, Mandad S, Rammner B, Jähne S, Salimi V, Koerbs C, Leibrandt R, Urlaub H, Schikorski T, Rizzoli SO
First Author	Helm MS
Last Author	Rizzoli SO

External Resources

14757https://www.addgene.org/14757 Addgene identifier (14757, reused)

gro-2/88345http://resolver.sub.uni-goettingen.de/purl?gro-2/88345 GRO.publications identifier

10116https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 NCBI taxonomy (rat, Rattus norvegicus)

0000-0002-6217-911Xhttps://orcid.org/0000-0002-6217-911X ORCID identifier (Martin S. Helm)

0000-0003-4757-9462https://orcid.org/0000-0003-4757-9462 ORCID identifier (Tal M. Dankovich)

0000-0002-1651-2292https://orcid.org/0000-0002-1651-2292 ORCID identifier (Sebastian Jähne)

0000-0003-1837-5233https://orcid.org/0000-0003-1837-5233 ORCID identifier (Henning Urlaub)

0000-0002-1667-7839https://orcid.org/0000-0002-1667-7839 ORCID identifier (Silvio-Olivier Rizzoli)

PXD015308http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD015308 Pride proteome dataset (PXD015308)

148https://sfb1190.med.uni-goettingen.de/production/literature/publications/148 Published Data Registry entry (CRC 1190)

125https://sfb1286.uni-goettingen.de/literature/publications/125 Published Data Registry entry (CRC 1286)

1/73https://mbexc.uni-goettingen.de/archive/1/73 Research Data Archive (supplemental material)

01rpmzy83https://ror.org/01rpmzy83 ROR identifier (01rpmzy83, Central University of the Caribbean)

03e76ya46https://ror.org/03e76ya46 ROR identifier (03e76ya46, Max Planck Institute for Biophysical Chemistry)

05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)

02kkvpp62https://ror.org/02kkvpp62 ROR identifier (02kkvpp62, Technical University of Munich)

021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)

01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)

SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

SCR_014237https://scicrunch.org/resolver/SCR_014237 SciCrunch identifier (RRID:SCR_014237, Huygens)

SCR_014627https://scicrunch.org/resolver/SCR_014627 SciCrunch identifier (RRID:SCR_014627, I-TASSER)

SCR_010587https://scicrunch.org/resolver/SCR_010587 SciCrunch identifier (RRID:SCR_010587, Icy)

SCR_014632https://scicrunch.org/resolver/SCR_014632 SciCrunch identifier (RRID:SCR_014632, IUPred)

SCR_001622https://scicrunch.org/resolver/SCR_001622 SciCrunch identifier (RRID:SCR_001622, MATLAB)

SCR_014485https://scicrunch.org/resolver/SCR_014485 SciCrunch identifier (RRID:SCR_014485, MaxQuant)

SCR_016137https://scicrunch.org/resolver/SCR_016137 SciCrunch identifier (RRID:SCR_016137, Microsoft Excel)

SCR_014329https://scicrunch.org/resolver/SCR_014329 SciCrunch identifier (RRID:SCR_014329, NIS-Elements)

SCR_008394https://scicrunch.org/resolver/SCR_008394 SciCrunch identifier (RRID:SCR_008394, Python)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM1_ESM.pdf Supplemental material

goeseek.gwdguser.de/studies/141https://goeseek.gwdguser.de/studies/141 Supplemental material (goeseek study)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM2_ESM.pdf Supplemental material (reporting summary)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM3_ESM.xlsx Supplemental material (table)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM4_ESM.xlsx Supplemental material (table)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM5_ESM.xlsx Supplemental material (table)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM6_ESM.xlsx Supplemental material (table)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM7_ESM.xlsx Supplemental material (table)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41593-021-00874-w/MediaObjects/41593_2021_874_MOESM8_ESM.xlsx Supplemental material (table)

mbexc.de...pines/https://mbexc.de/a-large-scale-nanoscopy-and-biochemistry-analysis-of-postsynaptic-dendritic-spines/ Website entry (mbexc.de)

Linked Antibodies

PID	Type	Antigen symbol	Antibody Registry ID	Name
primary-0006	primary	CALM1	AB_725815	Anti-Calmodulin 1/2/3 antibody
primary-0118	primary	SYP	AB_1210382	Anti-Synaptophysin 1 antibody
primary-0126	primary	SNAP91	AB_887691	Anti-AP 180 antibody
primary-0131	primary	DNM1	AB_397640	Purified Mouse Anti-Dynamin Antibody
primary-0134	primary	HSPA8	AB_627761	HSC 70 Antibody
primary-0137	primary	NSF	AB_887751	Anti-NSF antibody
primary-0138	primary	RAB3	AB_397762	Rab3 antibody
primary-0141	primary	SNAP23	AB_887788	Anti-SNAP 23 antibody
primary-0142	primary	SNAP25	AB_887794	Anti-SNAP 25 antibody
primary-0146	primary	SYT7	AB_887838	Anti-Synaptotagmin 7 antibody
primary-0148	primary	STX16	AB_887799	Anti-Syntaxin 16 antibody
primary-0150	primary	VAMP1	AB_887807	Anti-Synaptobrevin antibody
primary-0151	primary	VAMP2	AB_887811	Anti-Synaptobrevin 2 antibody
primary-0155	primary	VTI1A	AB_398752	Vti1a antibody
primary-0205	primary	RPS6	AB_331355	S6 Ribosomal Protein (5G10) Rabbit mAb
primary-0236	primary	MAP2	AB_2138183	Anti-MAP 2 antibody
primary-0334	primary	ANK3	AB_10673030	Anti-Ankyrin-G (Staining) Antibody
primary-0336	primary	SYT5/9	AB_2199639	Anti-Synaptotagmin 5/9 antibody
primary-0492	primary	HOMER1	AB_2120992	Anti-Homer 1 antibody
primary-0553	primary	HOMER1	AB_887730	Anti-Homer 1 antibody
primary-0554	primary	DLG4	AB_2292883	PSD95 (D27E11) XP® Rabbit mAb
primary-0592	primary	APP	AB_94882	Anti-APP A4 Antibody
primary-0688	primary	ADAM22		ADAM22 Antibody
primary-0689	primary	AKT1	AB_915783	Akt (pan) (C67E7) Rabbit mAb
primary-0690	primary	ARC	AB_887694	Arc antibody
primary-0691	primary	SPTBN2	AB_399853	Purified Mouse Anti-β-Spectrin II
primary-0692	primary	TUBB3	AB_10694505	β3-Tubulin (D71G9) XP Antibody
primary-0693	primary	BDNF	AB_10995734	BDNF antibody
primary-0694	primary	CALB1	AB_2068199	Calbindin D28k antibody
primary-0695	primary	PPP3CA	AB_2661875	Calcineurin A antibody
primary-0696	primary	CALR	AB_2688013	Calreticulin (D3E6) XP® Antibody
primary-0697	primary	CALB2	AB_11035704	Calretinin Antibody
primary-0698	primary	CAMK2B	AB_10675311	CaMKII monoclonal antibody
primary-0699	primary	CAPS	AB_1209327	Anti-CAPS1 antibody
primary-0700	primary	CACNA1D	AB_2340920	Anti-CaV1.3 Antibody
primary-0701	primary	CACNA1A	AB_2619841	Ca2+ channel P/Q-type alpha-1A antibody
primary-0702	primary	CDC42	AB_2539858	Cdc42 Polyclonal Antibody
primary-0703	primary	CHGA	AB_2619972	Chromogranin A antibody
primary-0704	primary	CHGB	AB_2619973	Chromogranin B antibody
primary-0705	primary	SCG2	AB_298964	Anti-Chromogranin C/SGII antibody
primary-0706	primary	CLTC	AB_397866	Clathrin Heavy Chain antibody
primary-0707	primary	CLTB	AB_887706	Clathrin light chain antibody
primary-0708	primary	CTTN	AB_2088122	Cortactin antibody
primary-0709	primary	DLGAP1	AB_11017331	GKAP/DLGAP1 Antibody
primary-0710	primary	DRD1	AB_732002	Anti-Dopamine Receptor D1 antibody
primary-0711	primary	DRD2	AB_2094980	Anti-Dopamine D2 Receptor Antibody
primary-0712	primary	DBN1	AB_2089489	Drebrin 1 Antibody
primary-0713	primary	PDIA4	AB_10622112	ERp72 (D70D12) XP® antibody
primary-0714	primary	GAD2	AB_2107894	GAD 2 / GAD 65 antibody
primary-0715	primary	GFAP	AB_2232308	GFAP antibody
primary-0716	primary	GRIK1	AB_2039896	Anti-GRIK1 (GluK1) (extracellular) Antibody
primary-0717	primary	GRIN1	AB_887750	GluN 1 antibody
primary-0718	primary	GRIN2A	AB_2315842	Anti-GluN2A/NR2A Antibody
primary-0719	primary	GRIN2B	AB_2232584	Anti-GluN2B/NR2B Glutamate Receptor Antibody
primary-0720	primary	GRIA1	AB_2113441	GluA1 antibody
primary-0721	primary	GRIA2	AB_2039881	Anti-GluR2 (GluA2) (extracellular) Antibody
primary-0722	primary	GRIA3	AB_2533059	GluR3 Monoclonal Antibody
primary-0723	primary	GRIP1	AB_10804287	GRIP antibody
primary-0724	primary	HOMER2	AB_10807099	Homer 2 antibody
primary-0725	primary	HOMER3	AB_10804288	Homer 3 antibody
primary-0726	primary	AIF1	AB_2493179	IBA1 antibody
primary-0727	primary	IGF1R	AB_2122378	IGF-I Receptor β Antibody
primary-0728	primary	KCNJ2	AB_11023524	Kir2.1 Antibody
primary-0729	primary	KCNA1	AB_10978519	KCNA1 Polyclonal Antibody
primary-0730	primary	KCNB1	AB_2131650	Kv2.1 antibody
primary-0731	primary	NGFR	AB_10839265	p75NTR (D4B3) XP® antibody
primary-0732	primary	CHRM1	AB_11021120	Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody
primary-0733	primary	GRM1	AB_2279521	Anti-mGluR1a antibody
primary-0734	primary	GRM2	AB_2715600	Recombinant Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody
primary-0735	primary	GRM5	AB_1523944	Recombinant Anti-Metabotropic Glutamate Receptor 5 antibody
primary-0736	primary	MYO5A	AB_260558	Anti-Myosin Va (LE-16) antibody
primary-0737	primary	SCN1B	AB_2341071	Anti-SCN1B (NaVβ1) (extracellular) Antibody
primary-0738	primary	ATP1A3	AB_2274447	ATP1A3 Monoclonal Antibody
primary-0739	primary	SCN1A	AB_310253	Anti-Na+ Channel alpha, cardiac (III-IV loop) antibody
primary-0740	primary	SCN3A	AB_2040007	Anti-SCN3A (NaV1.3) Antibody
primary-0741	primary	NEFH	AB_10948043	PathPlusTM Polyclonal Rabbit anti‑Rat NEFH / NF‑H Antibody
primary-0742	primary	NEFL	AB_887743	Neurofilament L antibody
primary-0743	primary	NOS1	AB_325021	nNOS Polyclonal Antibody
primary-0744	primary	OLIG2	AB_2620030	Olig 2 antibody
primary-0745	primary	PVALB	AB_10000344	Rabbit anti-parvalbumin antibody
primary-0746	primary	DLG2	AB_2533171	PSD93 Polyclonal Antibody
primary-0747	primary	RAB11A/RAB11B	AB_2253210	Rab11 Antibody
primary-0748	primary	RAB4	AB_398205	Rab4 antibody
primary-0749	primary	RAB7A	AB_1904103	Rab7 (D95F2) XP® Rabbit mAb
primary-0750	primary	RAB9A	AB_10621426	Rab9A (D52G8) XP® Rabbit mAb
primary-0751	primary	RAPSN	AB_10794196	Anti-RAPSN Antibody
primary-0752	primary	RPL7A	AB_2182061	Ribosomal Protein L7a (R225) Antibody
primary-0753	primary	RPS3	AB_10622028	Ribosomal Protein S3 (D50G7) XP® Rabbit mAb
primary-0754	primary	SEC22B	AB_993020	Sec 22b antibody
primary-0755	primary	SEPTIN7	AB_10601573	Anti-SEPT7 Antibody
primary-0756	primary	SHANK1	AB_2619859	Shank 1 antibody
primary-0757	primary	SHANK2	AB_2619860	Shank 2 antibody
primary-0758	primary	SHANK3	AB_2619862	Shank 3 antibody
primary-0759	primary	NEFM	AB_448147	Anti-200kDa + 160kDa Neurofilament antibody
primary-0760	primary	SNAP29	AB_887795	SNAP 29 antibody
primary-0761	primary	SNAP47	AB_887899	SNAP 47 antibody
primary-0762	primary	SYT4	AB_2619771	Synaptotagmin 4 antibody
primary-0763	primary	SYNGAP1	AB_2287112	SynGAP Polyclonal Antibody
primary-0764	primary	STX1A	AB_887844	Syntaxin 1 antibody
primary-0765	primary	STX2	AB_887850	Syntaxin 2 antibody
primary-0766	primary	STX3	AB_887851	Syntaxin 3 antibody
primary-0767	primary	STX4	AB_887853	Syntaxin 4 antibody
primary-0768	primary	STX5	AB_887800	Syntaxin 5 antibody
primary-0769	primary	STX6	AB_2196500	Syntaxin 6 (C34B2) Rabbit mAb
primary-0770	primary	STX8	AB_887855	Syntaxin 8 antibody
primary-0771	primary	TGOLN2	AB_796176	Anti-TGN38 antibody
primary-0772	primary	TOMM20	AB_1843992	Anti-TOMM20 antibody
primary-0773	primary	TFRC	AB_10673794	Anti-Transferrin Receptor antibody
primary-0774	primary	NTRK2	AB_778636	Anti-TrkB antibody
primary-0775	primary	SLC18A3	AB_887864	VAChT antibody
primary-0776	primary	VAMP7	AB_1271093	Anti-SYBL1/VAMP-7 antibody