Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0667 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0667 |
| Research group | RG Griesinger |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | GFAP |
| Antibody Registry ID(s) | AB_304558 |
| Name | Anti-GFAP antibody |
| Alternative name | intermediate filament protein |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Chicken |
| Reacts with | Mouse, Rat |
| Clone | |
| Isotype | IgY |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab4674 |
| Lot no. | |
| Description | IHC (PFA fixed) Use at an assay dependent concentration. IHC-FrFl 1/1000 - 1/5000. Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods. ICC Use a concentration of 1 mg/ml. IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa. Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band. |
| Localization | |
| Storage instruction | Shipped at 4°C. Store at +4°C. Do Not Freeze. |
| Receipt date | 2021-08-19 00:00:00 |
| Preparation date | 2021-08-19 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFAP |
| Wikipedia | http://en.wikipedia.org/wiki/GFAP |
| Uniprot | http://www.uniprot.org/uniprot/?query=GFAP |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFAP |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_304558 |
Applications
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Linked publications
Low-Expressing Synucleinopathy Mouse Models Based on Oligomer-Forming Mutations and C-Terminal Truncation of α-Synuclein
Martinez Hernandez A, Silbern I, Geffers I, Tatenhorst L, Becker S, Urlaub H, Zweckstetter M, Griesinger C, Eichele G
Front Neurosci 2021 : 10.3389/fnins.2021.643391
