Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0667 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0667 |
| Research group | RG Griesinger |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | GFAP |
| Antibody Registry ID(s) | AB_304558 |
| Name | Anti-GFAP antibody |
| Alternative name | intermediate filament protein |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Chicken |
| Reacts with | mouse, rat |
| Clone | |
| Isotype | IgY |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab4674 |
| Lot no. | |
| Description | IHC (PFA fixed) Use at an assay dependent concentration. IHC-FrFl 1/1000 - 1/5000. Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods. ICC Use a concentration of 1 mg/ml. IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa. Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band. |
| Localization | |
| Storage instruction | Shipped at 4°C. Store at +4°C. Do Not Freeze. |
| Receipt date | 2021-08-19 00:00:00 |
| Preparation date | 2021-08-19 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFAP |
| Wikipedia | http://en.wikipedia.org/wiki/GFAP |
| Uniprot | http://www.uniprot.org/uniprot/?query=GFAP |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFAP |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_304558 |
Applications
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Linked publications
Low-Expressing Synucleinopathy Mouse Models Based on Oligomer-Forming Mutations and C-Terminal Truncation of α-Synuclein
Martinez Hernandez A, Silbern I, Geffers I, Tatenhorst L, Becker S, Urlaub H, Zweckstetter M, Griesinger C, Eichele G
Front Neurosci 2021 : 10.3389/fnins.2021.643391
Astrocytes adopt a progenitor-like migratory strategy for regeneration in adult brain
Herwerth M, Wyss MT, Schmid NB, Condrau J, Ravotto L, Mateos Melero JM, Kaech A, Bredell G, Thomas C, Stadelmann C, Misgeld T, Bennett JL, Saab AS, Jessberger S, Weber B
bioRxiv 2024 : 10.1101/2024.05.18.594292
Lead as a toxic environmental toxicant in models of synucleinopathies
Shvachiy L, Amaro-Leal Â, Machado F, Rocha I, Geraldes V, Outeiro TF
bioRxiv 2024 : 10.1101/2024.10.10.617554
