[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"667","pid":"primary-0667","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0667","research_group":"RG Griesinger","lab_id":"","type":"primary","antigen_symbol":"GFAP","antibody_registry_id":"AB_304558","name":"Anti-GFAP antibody ","alternative_name":"intermediate filament protein","tag_flurophore":"","raised_id":"Chicken","reacts_with":"mouse, rat","clone":"","isotype":"IgY","clonality":"polyclonal","demasking":"unknown","antigen":"Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli.","company":"Abcam","catalog_no":"ab4674","lot_no":"","description":"IHC (PFA fixed) \t\t\r\nUse at an assay dependent concentration.\r\nIHC-FrFl \t\r\n1\/1000 - 1\/5000.\r\n\r\nTry this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.\r\nICC \t\t\r\nUse a concentration of 1 mg\/ml.\r\nIHC-P \t\r\n1\/200 - 1\/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.\r\nWB \t\r\n1\/1000 - 1\/5000. Predicted molecular weight: 50 kDa.\r\n\r\nExpect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.","last_modified":"Klauenberg, Vanessa"}]