Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0624 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0624 |
| Research group | RG Wollnik |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | POU5F1 |
| Antibody Registry ID(s) | AB_628051 |
| Name | Anti-Oct-3/4 Antibody |
| Alternative name | MGC22487 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | Mouse, Rat, Human |
| Clone | C-10 |
| Isotype | IgG2b |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | raised against amino acids 1-134 of Oct-3/4 of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-5279 |
| Lot no. | |
| Description | Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500), immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500), flow cytometry (1 μg per 1 x 106cells) and solid phase ELISA (starting dilution 1:30, dilutionrange 1:30-1:3000) |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required. |
| Receipt date | 2021-07-29 00:00:00 |
| Preparation date | 2021-07-29 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=POU5F1 |
| Wikipedia | http://en.wikipedia.org/wiki/POU5F1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=POU5F1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=POU5F1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_628051 |
Applications
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Linked publications
Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y, Rehimi R, Kayserili H, Brühl J, Sofiadis K, Zirkel A, Palikyras S, Mizi A, Li Y, Yigit G, Hoischen A, Frank S, Russ N, Trautwein J, van Bon B, Gilissen C, Laugsch M, Gusmao EG, Josipovic N, Altmüller J, Nürnberg P, Längst G, Kaiser FJ, Watrin E, Brunner H, Rada-Iglesias A, Kurian L, Wollnik B, Bouazoune K, Papantonis A
Nat Commun 2021 : 10.1038/s41467-021-23327-1
Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schöler HR
eLife 2022 : 10.7554/elife.71533
