Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0367 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0367 |
| Research group | RG Fernandez-Busnadiego |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | SNCA |
| Antibody Registry ID(s) | AB_869973 |
| Name | Recombinant Anti-Alpha-synuclein (phospho S129) antibody |
| Alternative name | Alpha synuclein antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Mouse, Rat, Human |
| Clone | EP1536Y |
| Isotype | IgG |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide within Human Alpha-synuclein aa 100 to the C-terminus. The exact sequence is proprietary. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab51253 |
| Lot no. | |
| Description | IHC-FrFl Use at an assay dependent concentration. WB 1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. Dot blot Use at an assay dependent concentration. ELISA Use at an assay dependent concentration. IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Localization | Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. |
| Storage instruction | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Receipt date | 2021-04-20 00:00:00 |
| Preparation date | 2021-04-20 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=SNCA |
| Wikipedia | http://en.wikipedia.org/wiki/SNCA |
| Uniprot | http://www.uniprot.org/uniprot/?query=SNCA |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=SNCA |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_869973 |
Applications
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Linked publications
In situ architecture of neuronal α-Synuclein inclusions
Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJ, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R
bioRxiv 2020 : 10.1101/2020.08.07.234138
RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies
Koss DJ, Bondarevaite O, Adams S, Leite M, Giorgini F, Attems J, Outeiro TF
Brain Pathol 2020 : 10.1111/bpa.12890
Cofilin pathology is a new player on α-synuclein-induced spine impairment in models of hippocampal synucleinopathy
Oliveira da Silva MI, Santejo M, Babcock I, Magalhães A, Minamide L, Castillo E, Gerhardt E, Fahlbusch C, Swanson R, Outeiro T, Bamburg J, Liz M
bioRxiv 2021 : 10.1101/2021.02.02.425931
Cerebral Dopamine Neurotrophic Factor Reduces α-Synuclein Aggregation and Propagation and Alleviates Behavioural Alterations in vivo
Albert K, Raymundo DP, Panhelainen A, Eesmaa A, Shvachiy L, Araújo GR, Chmielarz P, Yan X, Singh A, Cordeiro Y, Palhano FL, Foguel D, Luk KC, Domanskyi A, Voutilainen MH, Huttunen HJ, Outeiro TF, Saarma M, Almeida MS, Airavaara M
Mol Ther 2021 : 10.1016/j.ymthe.2021.04.035
In situ architecture of neuronal α-Synuclein inclusions
Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJB, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R
Nat Commun 2021 : 10.1038/s41467-021-22108-0
Brain iron enrichment attenuates α‐synuclein spreading after injection of preformed fibrils
Joppe KDn, Tatenhorst L, Caldi Gomes L, Zhang S, Parvaz M, Carboni E, Roser A, El DeBakey H, Bähr M, Vogel‐Mikuš K, Ip CW, Becker S, Zweckstetter M, Lingor P
J Neurochem 2021 : 10.1111/jnc.15461
Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825
Prion-like α-synuclein pathology in the brain of infants with Krabbe disease
Hatton C, Ghanem SS, Koss DJ, Abdi IY, Gibbons E, Guerreiro R, Bras J, Walker L, Gelpi E, Heywood W, Outeiro TF, Attems J, McFarland R, Forsyth R, El-Agnaf OM, Erskine D
Brain 2022 : 10.1093/brain/awac002
Monitoring Alpha-Synuclein – Tau Interactions In Vitro and In Vivo Using Bimolecular Fluorescence Complementation
Torres-Garcia L, Domingues JM, Brandi E, Haikal C, Brás IC, Gerhardt E, Li W, Svanbergsson A, Outeiro TF, Gouras GK, Li J
Researchsquare 2021 : 10.21203/rs.3.rs-960446/v1
Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation
Torres-Garcia L, P. Domingues JM, Brandi E, Haikal C, Mudannayake JM, Brás IC, Gerhardt E, Li W, Svanbergsson A, Outeiro TF, Gouras GK, Li J
Sci Rep 2022 : 10.1038/s41598-022-06846-9
Glycation modulates alpha-synuclein fibrillization kinetics: a sweet spot for inhibition
Farzadfard A, König A, Petersen SV, Nielsen J, Vasili E, Dominguez-Meijide A, Buell AK, Outeiro TF, Otzen DE
J Biol Chem 2022 : 10.1016/j.jbc.2022.101848
The small aromatic compound SynuClean-D inhibits the aggregation and seeded polymerization of multiple α-synuclein strains
Peña-Díaz S, Pujols J, Vasili E, Pinheiro F, Santos J, Manglano-Artuñedo Z, Outeiro TF, Ventura S
J Biol Chem 2022 : 10.1016/j.jbc.2022.101902
Nuclear alpha-synuclein is present in the human brain and is modified in dementia with Lewy bodies
Koss DJ, Erskine D, Porter A, Palmoski P, Leite M, Attems J, Outeiro TF
bioRxiv 2021 : 10.1101/2021.10.20.465125
α-Synuclein triggers cofilin pathology and dendritic spine impairment via a PrPC-CCR5 dependent pathway
Oliveira da Silva MI, Santejo M, Babcock IW, Magalhães A, Minamide LS, Won S, Castillo E, Gerhardt E, Fahlbusch C, Swanson RA, Outeiro TF, Taipa R, Ruff M, Bamburg JR, Liz MA
Cell Death Dis 2024 : 10.1038/s41419-024-06630-9
