| Antigen symbol |
SNCA |
| Antibody Registry ID(s) |
AB_869973 |
| Name |
Recombinant Anti-Alpha-synuclein (phospho S129) antibody |
| Alternative name |
Alpha synuclein antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, human |
| Clone |
EP1536Y |
| Isotype |
IgG |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide within Human Alpha-synuclein aa 100 to the C-terminus. The exact sequence is proprietary. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab51253 |
| Lot no. |
|
| Description |
IHC-FrFl Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Localization |
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. |
| Storage instruction |
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Receipt date |
2021-04-20 00:00:00 |
| Preparation date |
2021-04-20 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |