Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0257 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0257 |
| Research group | RG Enderlein |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | CLTC |
| Antibody Registry ID(s) | AB_2083165 |
| Name | Anti-Clathrin heavy chain antibody |
| Alternative name | CHC17 antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | mouse, rat, human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human Clathrin heavy chain. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab21679 |
| Lot no. | |
| Description | WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). ICC Use a concentration of 1 µg/ml. |
| Localization | Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-03-31 00:00:00 |
| Preparation date | 2021-03-31 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=CLTC |
| Wikipedia | http://en.wikipedia.org/wiki/CLTC |
| Uniprot | http://www.uniprot.org/uniprot/?query=CLTC |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=CLTC |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2083165 |
Applications
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Linked publications
Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
Thiele JC, Helmerich DA, Oleksiievets N, Tsukanov R, Butkevich E, Sauer M, Nevskyi O, Enderlein J
ACS Nano 2020 : 10.1021/acsnano.0c07322
Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
bioRxiv 2021 : 10.1101/2021.12.20.473473
Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
Sci Adv 2022 : 10.1126/sciadv.abo2506
Mouse oocytes sequester aggregated proteins in degradative super-organelles
Zaffagnini G, Cheng S, Salzer MC, Pernaute B, Duran JM, Irimia M, Schuh M, Böke E
Cell 2024 : 10.1016/j.cell.2024.01.031
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
Mougios N, Cotroneo ER, Imse N, Setzke J, Rizzoli SO, Simeth NA, Tsukanov R, Opazo F
Nat Commun 2024 : 10.1038/s41467-024-53030-w
