View primary antibody

Basic data

PID primary-0507
EPIC PID
Research group RG Fernandez-Busnadiego
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol FLAG
Antibody Registry ID(s) AB_259529
Name Monoclonal ANTI-FLAG® M2 antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised in
Reacts with all
Clone M2
Isotype IgG1
Clonality monoclonal
Demasking unknown
Antigen FLAG; peptide sequence DYKDDDDK
Crafted By company
Company / Manufacturer Sigma-Aldrich
Catalog no. F3165
Lot no.
Description western blot: 10 μg/mL (Protein A)
Localization
Storage instruction Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended
Receipt date 2021-05-12 00:00:00
Preparation date 2021-05-12 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

External links

Antibodypedia -
HGNC https://www.genenames.org/tools/search/#!/?query=FLAG
Wikipedia https://en.wikipedia.org/wiki/FLAG
Uniprot https://www.uniprot.org/uniprot/?query=FLAG
GeneCards https://www.genecards.org/cgi-bin/carddisp.pl?gene=FLAG
Antibody Registry https://antibodyregistry.org/search?q=AB_259529

Applications

No application comments yet.

Linked publications

PolyQ expansion does not alter the Huntingtin-HAP40 complex
Huang B et al.
bioRxiv 2021: 10.1101/2021.02.02.429316
 
Pathological polyQ expansion does not alter the conformation of the Huntingtin-HAP40 complex
Huang B et al.
Structure 2021: 10.1016/j.str.2021.04.003
 
Mapping protein interactions in the active TOM-TIM23 supercomplex
Gomkale R et al.
Nat Commun 2021: 10.1038/s41467-021-26016-1
 
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors
Dennerlein S et al.
eLife 2021: 10.7554/elife.68213
 
The RNA methyltransferase METTL8 installs m3C32 in mitochondrial tRNAsThr/Ser(UCN) to optimise tRNA structure and mitochondrial translation
Kleiber N et al.
Nat Commun 2022: 10.1038/s41467-021-27905-1
 
Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment
Cheng S et al.
Science 2022: 10.1126/science.abq4835
 
Drosophila MIC10b can polymerize into cristae-shaping filaments
Stephan T et al.
Life Sci Alliance 2024: 10.26508/lsa.202302177
 
A metabolically controlled contact site between vacuoles and lipid droplets in yeast
Diep DTV et al.
Dev Cell 2024: 10.1016/j.devcel.2024.01.016
 
The SecM arrest peptide traps a pre-peptide bond formation state of the ribosome
Gersteuer F et al.
Nat Commun 2024: 10.1038/s41467-024-46762-2
 
Guanosine 10 methylation by TRMT11–TRMT112 optimizes human mitochondrial tRNAs for efficient translation
Petrosyan J et al.
Nucleic Acids Res 2026: 10.1093/nar/gkag859