| Antigen symbol |
TUBA4A |
| Antibody Registry ID(s) |
AB_2241126 |
| Name |
Anti-alpha Tubulin antibody |
| Alternative name |
ALS22 antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, human |
| Clone |
DM1A |
| Isotype |
IgG1 |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Full length native protein (purified) corresponding to Chicken alpha Tubulin. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab7291 |
| Lot no. |
|
| Description |
<a href="https://rdp.sfb1565.uni-goettingen.de/antibodies/primary/primary-0011">sfb1565: primary-0011</a>
Flow Cyt Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking |
| Localization |
Cytoplasm > cytoskeleton. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2021-01-01 00:00:00 |
| Preparation date |
2021-03-23 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
|