View primary antibody

Basic data

PIDprimary-1048
EPIC PIDhttps://hdl.handle.net/21.11124/primary-1048
Research groupRG Cramer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTP53
Antibody Registry ID(s)AB_628082
NameAnti-p53 Antibody (DO-1)
Alternative nameLFS1
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withmouse, rat, human
CloneDO-1
IsotypeIgG2a κ
Clonalitymonoclonal
Demaskingunknown
AntigenN-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-126
Lot no.
DescriptionWestern Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 μg per 1 x 106 cells).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
Receipt date2022-08-02 00:00:00
Preparation date2022-08-02 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Sequence determinants of human gene regulatory elements
Sahu B, Hartonen T, Pihlajamaa P, Wei B, Dave K, Zhu F, Kaasinen E, Lidschreiber K, Lidschreiber M, Daub CO, Cramer P, Kivioja T, Taipale J
Nat Genet 2022 : 10.1038/s41588-021-01009-4