Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0852 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0852 |
| Research group | RG Fernandez-Busnadiego |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | GFP |
| Antibody Registry ID(s) | AB_303395 |
| Name | Anti-GFP antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | speciesIndependent |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab290 |
| Lot no. | |
| Description | sfb1565: primary-0015
ELISA Use at an assay dependent concentration. IHC-Fr Use at an assay dependent concentration. Reported to work at dilutions up to 1/3000. Use secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab15077). ICC 1/200 - 1/1000. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody. IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IP Use at an assay dependent concentration. Use at 1µl per 10cm tissue culture dish (use 10µl protein A agarose CL4B to precipitate the immune complex). WB 1/1000 - 1/2500. It is recommended to use 12.5% SDS-PAGE and to transfer to PVDF membrane. Use 1x Blotto (or 3% BSA in PBS) for diluting and blocking. Use PBS in 3x 5min washing steps throughout the immunolabelling. Probe with ab290 at 1:1000 - 1:5000 dilution and use Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1:5000 dilution with ECL detection method. ab290 has been reported to work at 1:50,000 and dilutions around this range should be tested if high background is seen. Both incubation steps should be for 1hr at 22°C. IHC-FoFr 1/200 - 1/500. IHC-FrFl Use at an assay dependent concentration. Electron Microscopy 1/1000 - 1/4000. |
| Localization | |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-11-01 00:00:00 |
| Preparation date | 2021-11-23 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFP |
| Wikipedia | http://en.wikipedia.org/wiki/GFP |
| Uniprot | http://www.uniprot.org/uniprot/?query=GFP |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFP |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_303395 |
Applications
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Linked publications
Cnm1 mediates nucleus–mitochondria contact site formation in response to phospholipid levels
Eisenberg-Bord M, Zung N, Collado J, Drwesh L, Fenech EJ, Fadel A, Dezorella N, Bykov YS, Rapaport D, Fernandez-Busnadiego R, Schuldiner M
J Cell Biol 2021 : 10.1083/jcb.202104100
In-situ architecture of the human prohibitin complex
Lange F, Ratz M, Dohrke J, Wenzel D, Ilgen P, Riedel D, Jakobs S
bioRxiv 2024 : 10.1101/2024.02.14.579514
The molecular mechanism of on-demand sterol biosynthesis at organelle contact sites
Zung N, Aravindan N, Boshnakovska A, Valenti R, Preminger N, Jonas F, Yaakov G, Willoughby MM, Homberg B, Keller J, Kupervaser M, Dezorella N, Dadosh T, Wolf SG, Itkin M, Malitsky S, Brandis A, Barkai N, Fernández-Busnadiego R, Reddi AR, Rehling P, Rapaport D, Schuldiner M
bioRxiv 2024 : 10.1101/2024.05.09.593285
