[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"852","pid":"primary-0852","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0852","research_group":"RG Fernandez-Busnadiego","lab_id":"","type":"primary","antigen_symbol":"GFP","antibody_registry_id":"AB_303395","name":"Anti-GFP antibody","alternative_name":"","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"speciesIndependent","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria.","company":"Abcam","catalog_no":"ab290","lot_no":"","description":"\u0026lt;a href=\u0026quot;https:\/\/rdp.sfb1565.uni-goettingen.de\/antibodies\/primary\/primary-0015\u0026quot;\u0026gt;sfb1565: primary-0015\u0026lt;\/a\u0026gt;\r\n\u0026lt;br\u0026gt;ELISA\t\t\r\nUse at an assay dependent concentration.\r\n\r\nIHC-Fr\t\t\r\nUse at an assay dependent concentration. Reported to work at dilutions up to 1\/3000. Use secondary antibody Goat Anti-Rabbit IgG H\u0026amp;L (Alexa Fluor\u00ae 488) (ab15077). \r\n\r\nICC\t\t\r\n1\/200 - 1\/1000. We recommend Goat Anti-Rabbit IgG H\u0026amp;L (Alexa Fluor\u00ae 488) (ab150081) secondary antibody.\r\n\r\nIHC-P\t\r\n1\/500 - 1\/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.\r\n\r\nIP\t\r\nUse at an assay dependent concentration. Use at 1\u00b5l per 10cm tissue culture dish (use 10\u00b5l protein A agarose CL4B to precipitate the immune complex).\r\n\r\nWB\t\r\n1\/1000 - 1\/2500. It is recommended to use 12.5% SDS-PAGE and to transfer to PVDF membrane. Use 1x Blotto (or 3% BSA in PBS) for diluting and blocking. Use PBS in 3x 5min washing steps throughout the immunolabelling. Probe with ab290 at 1:1000 - 1:5000 dilution and use Goat Anti-Rabbit IgG H\u0026amp;L (HRP) (ab205718) at 1:5000 dilution with ECL detection method. ab290 has been reported to work at 1:50,000 and dilutions around this range should be tested if high background is seen. Both incubation steps should be for 1hr at 22\u00b0C.\r\n\r\nIHC-FoFr\t\t\r\n1\/200 - 1\/500.\r\n\r\nIHC-FrFl\t\t\r\nUse at an assay dependent concentration.\r\n\r\nElectron Microscopy\t\t\r\n1\/1000 - 1\/4000.","last_modified":", "}]