View primary antibody

Basic data

PIDprimary-0786
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0786
Research groupRG Gollisch
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolAIF1
Antibody Registry ID(s)AB_2636859
NameRecombinant Anti-Iba1 antibody
Alternative nameinterferon gamma responsive transcript
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Rat, Human
CloneEPR16588
IsotypeIgG
Clonalitymonoclonal
Demaskingunknown
AntigenSynthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab178846
Lot no.
DescriptionFlow Cyt (Intra) 1/160. IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. WB 1/500 - 1/2000. Detects a band of approximately 10, 15 kDa (predicted molecular weight: 17 kDa). Abcam recommends blocking in 3% milk for cleanest results in WB. Blocking with BSA gives slightly higher background. ICC/IF 1/500.
LocalizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date2021-10-04 00:00:00
Preparation date2021-10-04 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825