View primary antibody

Basic data

PIDprimary-0785
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0785
Research groupRG Gollisch
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolNOS2
Antibody Registry ID(s)AB_310179
NameAnti-iNOS/NOS II Antibody
Alternative nameHEP-NOS
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withmouse, bovine, sheep, guineaPig
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenPartial fusion protein raised against the N-terminus of murine iNOS/NOS II.
Crafted Bycompany
Company / ManufacturerUpstate (Millipore)
Catalog no.06-573
Lot no.
DescriptionImmunohistochemistry: A previous lot of this antibody has been reported to detect iNOS/NOS II in 2% paraformaldehyde-fixed sheep lung (Sherman, T.S., 1999). Immunoprecipitation: 4 μg of a previous lot immunoprecipitated iNOS/NOS II from 500 μg of L929B cells which had been stimulated with 10 ng/mL IFNg and 1 μg/mL LPS.
Localization
Storage instructionStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Receipt date2021-10-04 00:00:00
Preparation date2021-10-04 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825