View primary antibody

Basic data

PIDprimary-0776
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0776
Research groupRG Rizzoli
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolVAMP7
Antibody Registry ID(s)AB_1271093
NameAnti-SYBL1/VAMP-7 antibody
Alternative nameTI-VAMP
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Rat, Human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenA synthetic peptide as a part of human (and other species) SYBL1 conjugated to an immunogenic carrier protein
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab68776
Lot no.
DescriptionWB 1/300 - 1/3000. Predicted molecular weight: 24 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. IHC-Fr 1/300 - 1/3000. IHC-P 1/300 - 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Anti-SYBL1/VAMP-7 antibody (ab68776) is not available anymore
LocalizationCytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date2021-10-01 00:00:00
Preparation date2021-10-01 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines
Helm MS, Dankovich TM, Mandad S, Rammner B, Jähne S, Salimi V, Koerbs C, Leibrandt R, Urlaub H, Schikorski T, Rizzoli SO
Nat Neurosci 2021 : 10.1038/s41593-021-00874-w