View primary antibody

Basic data

PIDprimary-0773
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0773
Research groupRG Rizzoli
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTFRC
Antibody Registry ID(s)AB_10673794
NameAnti-Transferrin Receptor antibody
Alternative nameCD71
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide corresponding to Human Transferrin Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab84036
Lot no.
DescriptionWB Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa). ICC Use a concentration of 5 µg/ml. IHC-P 1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Flow Cyt Use 1µg for 106 cells.
LocalizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-09-29 00:00:00
Preparation date2021-09-29 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines
Helm MS, Dankovich TM, Mandad S, Rammner B, Jähne S, Salimi V, Koerbs C, Leibrandt R, Urlaub H, Schikorski T, Rizzoli SO
Nat Neurosci 2021 : 10.1038/s41593-021-00874-w