View primary antibody

Basic data

PIDprimary-0684
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0684
Research groupRG Gollisch
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolMAP2
Antibody Registry ID(s)AB_297885
NameAnti-MAP2 antibody
Alternative nameDKFZp686I2148 antibody
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withRat
CloneHM-2
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenFull length protein corresponding to Rat MAP2. Rat brain microtubule associated proteins (MAPs).
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab11267
Lot no.
DescriptionICC Use a concentration of 2 µg/ml. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.
LocalizationCytoplasm, cytoskeleton.
Storage instructionShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Receipt date2021-09-02 00:00:00
Preparation date2021-09-02 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825

Ectosomes and exosomes are distinct proteomic entities that modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Riedel D, Parfentev I, Gerhardt E, Urlaub H, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.06.24.449731