Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0682 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0682 |
| Research group | RG Gollisch |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | AIF1 |
| Antibody Registry ID(s) | AB_2224402 |
| Name | Anti-Iba1 antibody |
| Alternative name | interferon gamma responsive transcript |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Goat |
| Reacts with | Rat, Human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal) |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab5076 |
| Lot no. | |
| Description | IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa). A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
| Localization | Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. |
| Storage instruction | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Receipt date | 2021-08-31 00:00:00 |
| Preparation date | 2021-08-31 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=AIF1 |
| Wikipedia | http://en.wikipedia.org/wiki/AIF1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=AIF1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=AIF1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2224402 |
Applications
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Linked publications
Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells
Brás IC, Khani MH, Vasili E, Möbius W, Riedel D, Parfentev I, Gerhardt E, Fahlbusch C, Urlaub H, Zweckstetter M, Gollisch T, Outeiro TF
bioRxiv 2021 : 10.1101/2021.07.18.452825
White matter integrity requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM, Weil M, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave K, Möbius W
bioRxiv 2020 : 10.1101/2020.09.02.279612
White matter integrity in mice requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave KA, Möbius W
Nat Commun 2022 : 10.1038/s41467-022-28720-y
