View primary antibody

Basic data

PIDprimary-0630
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0630
Research groupRG Wollnik
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBB3
Antibody Registry ID(s)AB_444319
NameAnti-beta III Tubulin antibody
Alternative nameCFEOM3
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Rat, Human, Marmoset, Dogfish, Catshark
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab18207
Lot no.
DescriptionFlow Cyt Use 0.01µg for 106 cells. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ICC/IF Use a concentration of 1 - 5 µg/ml. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody WB Use a concentration of 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta III Tubulin peptide (ab18660).
LocalizationCytoplasm > cytoskeleton.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-07-29 00:00:00
Preparation date2021-07-29 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y, Rehimi R, Kayserili H, Brühl J, Sofiadis K, Zirkel A, Palikyras S, Mizi A, Li Y, Yigit G, Hoischen A, Frank S, Russ N, Trautwein J, van Bon B, Gilissen C, Laugsch M, Gusmao EG, Josipovic N, Altmüller J, Nürnberg P, Längst G, Kaiser FJ, Watrin E, Brunner H, Rada-Iglesias A, Kurian L, Wollnik B, Bouazoune K, Papantonis A
Nat Commun 2021 : 10.1038/s41467-021-23327-1