Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0629 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0629 |
| Research group | RG Wollnik |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | MYH1E |
| Antibody Registry ID(s) | AB_2147781 |
| Name | MF 20 antibody |
| Alternative name | MYO-1 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | Amphibian, Avian, Chicken, Fish, Human, Lizard, Mammal, Pig, Snake, Xenopus, Zebrafish |
| Clone | |
| Isotype | MIgG2b |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Myosin heavy chain, sarcomere (MHC) |
| Crafted By | company |
| Company / Manufacturer | DSHB |
| Catalog no. | MF20 |
| Lot no. | |
| Description | Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml. |
| Localization | |
| Storage instruction | Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
| Receipt date | 2021-07-29 00:00:00 |
| Preparation date | 2021-07-29 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=MYH1E |
| Wikipedia | http://en.wikipedia.org/wiki/MYH1E |
| Uniprot | http://www.uniprot.org/uniprot/?query=MYH1E |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=MYH1E |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2147781 |
Applications
No application comments yet.
Linked publications
Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y, Rehimi R, Kayserili H, Brühl J, Sofiadis K, Zirkel A, Palikyras S, Mizi A, Li Y, Yigit G, Hoischen A, Frank S, Russ N, Trautwein J, van Bon B, Gilissen C, Laugsch M, Gusmao EG, Josipovic N, Altmüller J, Nürnberg P, Längst G, Kaiser FJ, Watrin E, Brunner H, Rada-Iglesias A, Kurian L, Wollnik B, Bouazoune K, Papantonis A
Nat Commun 2021 : 10.1038/s41467-021-23327-1
