| Antigen symbol |
MYH1E |
| Antibody Registry ID(s) |
AB_2147781 |
| Name |
MF 20 antibody |
| Alternative name |
MYO-1 |
| Lab ID |
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| Tag / Fluorophore |
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| Raised in |
|
| Reacts with |
amphibian, avian, chicken, fish, human, lizard, mammal, pig, snake, Xenopus, zebrafish |
| Clone |
|
| Isotype |
MIgG2b |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Myosin heavy chain, sarcomere (MHC) |
| Crafted By |
company |
| Company / Manufacturer |
DSHB |
| Catalog no. |
MF20 |
| Lot no. |
|
| Description |
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml. |
| Localization |
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| Storage instruction |
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
| Receipt date |
2021-07-29 00:00:00 |
| Preparation date |
2021-07-29 00:00:00 |
| Created by |
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| Last modified |
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