View primary antibody

Basic data

PIDprimary-0625
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0625
Research groupRG Wollnik
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbol PAX3
Antibody Registry ID(s)AB_2722285
NamePAX3 Antibody
Alternative nameHUP2
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withHuman
Clone
IsotypeMIgG1
Clonalitymonoclonal
Demaskingunknown
Antigen Recombinant protein
Crafted Bycompany
Company / ManufacturerDSHB
Catalog no.PCRP-PAX3-1B3
Lot no.
DescriptionAlthough the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instructionAlthough many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date2021-07-29 00:00:00
Preparation date2021-07-29 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y, Rehimi R, Kayserili H, Brühl J, Sofiadis K, Zirkel A, Palikyras S, Mizi A, Li Y, Yigit G, Hoischen A, Frank S, Russ N, Trautwein J, van Bon B, Gilissen C, Laugsch M, Gusmao EG, Josipovic N, Altmüller J, Nürnberg P, Längst G, Kaiser FJ, Watrin E, Brunner H, Rada-Iglesias A, Kurian L, Wollnik B, Bouazoune K, Papantonis A
Nat Commun 2021 : 10.1038/s41467-021-23327-1