View primary antibody

Basic data

PIDprimary-0613
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0613
Research groupRG Wollnik
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolH3K27me3
Antibody Registry ID(s)AB_2561020
NameHistone H3K27me3 antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withhuman, mouse
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
Antigenraised against a peptide including trimethyl-lysine 27 of histone H3. testing
Crafted Bycompany
Company / ManufacturerActive Motif
Catalog no.39155
Lot no.
DescriptionApplications Validated by Active Motif: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 µg each ICC/IF: 2 µg/ml dilution IHC(FFPE): 2 µg/ml dilution WB*: 0.5 - 2 µg/ml dilution CUT&Tag: 1 µg per 50 µl reaction*
Localization
Storage instructionAvoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Receipt date2021-07-28 00:00:00
Preparation date2021-07-28 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

External links

Applications


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Linked publications


Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y, Rehimi R, Kayserili H, Brühl J, Sofiadis K, Zirkel A, Palikyras S, Mizi A, Li Y, Yigit G, Hoischen A, Frank S, Russ N, Trautwein J, van Bon B, Gilissen C, Laugsch M, Gusmao EG, Josipovic N, Altmüller J, Nürnberg P, Längst G, Kaiser FJ, Watrin E, Brunner H, Rada-Iglesias A, Kurian L, Wollnik B, Bouazoune K, Papantonis A
Nat Commun 2021 : 10.1038/s41467-021-23327-1