View primary antibody

Basic data

PIDprimary-0592
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0592
Research groupRG Gärtner
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolAPP
Antibody Registry ID(s)AB_94882
NameAnti-APP A4 Antibody
Alternative namepeptidase nexin-II
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withCanine, Human, Mouse, Monkey, Pig, Rat, Fish
Clone22C11
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenPurified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.
Crafted Bycompany
Company / ManufacturerMerck Millipore
Catalog no.MAB348
Lot no.
DescriptionImmunofluorescence Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20). Western blot: reducing conditions, 10μg/ml. Optimal working dilutions and protocols must be determined by the end user.
Localization
Storage instructionMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Receipt date2021-07-12 00:00:00
Preparation date2021-07-12 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Concurrent axon and myelin destruction differentiates X-linked adrenoleukodystrophy from multiple sclerosis
Bergner CG, Genc N, Hametner S, Franz J, Meer F, Mitkovski M, Weber MS, Stoltenburg‐Didinger G, Kühl J, Köhler W, Brück W, Gärtner J, Stadelmann C
Glia 2021 : 10.1002/glia.24042

A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines
Helm MS, Dankovich TM, Mandad S, Rammner B, Jähne S, Salimi V, Koerbs C, Leibrandt R, Urlaub H, Schikorski T, Rizzoli SO
Nat Neurosci 2021 : 10.1038/s41593-021-00874-w

White matter integrity requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM, Weil M, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave K, Möbius W
bioRxiv 2020 : 10.1101/2020.09.02.279612

White matter integrity in mice requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave KA, Möbius W
Nat Commun 2022 : 10.1038/s41467-022-28720-y