View primary antibody

Basic data

PIDprimary-0507
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0507
Research groupRG Fernandez-Busnadiego
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolFLAG
Antibody Registry ID(s)AB_259529
NameMonoclonal ANTI-FLAG® M2 antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withAll
CloneM2
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenFLAG; peptide sequence DYKDDDDK
Crafted Bycompany
Company / ManufacturerSigma-Aldrich
Catalog no.F3165
Lot no.
Descriptionwestern blot: 10 μg/mL (Protein A)
Localization
Storage instructionStore the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended
Receipt date2021-05-12 00:00:00
Preparation date2021-05-12 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

External links

Applications


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Linked publications


PolyQ expansion does not alter the Huntingtin-HAP40 complex
Huang B, Guo Q, Niedermeier ML, Cheng J, Engler T, Maurer M, Pautsch A, Baumeister W, Stengel F, Kochanek S, Fernández-Busnadiego R
bioRxiv 2021 : 10.1101/2021.02.02.429316

Pathological polyQ expansion does not alter the conformation of the Huntingtin-HAP40 complex
Huang B, Guo Q, Niedermeier ML, Cheng J, Engler T, Maurer M, Pautsch A, Baumeister W, Stengel F, Kochanek S, Fernández-Busnadiego R
Structure 2021 : 10.1016/j.str.2021.04.003

Mapping protein interactions in the active TOM-TIM23 supercomplex
Gomkale R, Linden A, Neumann P, Schendzielorz AB, Stoldt S, Dybkov O, Kilisch M, Schulz C, Cruz-Zaragoza LD, Schwappach B, Ficner R, Jakobs S, Urlaub H, Rehling P
Nat Commun 2021 : 10.1038/s41467-021-26016-1

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors
Dennerlein S, Poerschke S, Oeljeklaus S, Wang C, Richter-Dennerlein R, Sattmann J, Bauermeister D, Hanitsch E, Stoldt S, Langer T, Jakobs S, Warscheid B, Rehling P
eLife 2021 : 10.7554/elife.68213

The RNA methyltransferase METTL8 installs m3C32 in mitochondrial tRNAsThr/Ser(UCN) to optimise tRNA structure and mitochondrial translation
Kleiber N, Lemus-Diaz N, Stiller C, Heinrichs M, Mai MM, Hackert P, Richter-Dennerlein R, Höbartner C, Bohnsack KE, Bohnsack MT
Nat Commun 2022 : 10.1038/s41467-021-27905-1

Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment
Cheng S, Altmeppen G, So C, Welp LM, Penir S, Ruhwedel T, Menelaou K, Harasimov K, Stützer A, Blayney M, Elder K, Möbius W, Urlaub H, Schuh M
Science 2022 : 10.1126/science.abq4835

Drosophila MIC10b can polymerize into cristae-shaping filaments
Stephan T, Stoldt S, Barbot M, Carney TD, Lange F, Bates M, Bou Dib P, Inamdar K, Shcherbata HR, Meinecke M, Riedel D, Dennerlein S, Rehling P, Jakobs S
Life Sci Alliance 2024 : 10.26508/lsa.202302177

A metabolically controlled contact site between vacuoles and lipid droplets in yeast
Diep DTV, Collado J, Hugenroth M, Fausten RM, Percifull L, Wälte M, Schuberth C, Schmidt O, Fernández-Busnadiego R, Bohnert M
Dev Cell 2024 : 10.1016/j.devcel.2024.01.016

The SecM arrest peptide traps a pre-peptide bond formation state of the ribosome
Gersteuer F, Morici M, Gabrielli S, Fujiwara K, Safdari HA, Paternoga H, Bock LV, Chiba S, Wilson DN
Nat Commun 2024 : 10.1038/s41467-024-46762-2