View primary antibody

Basic data

PIDprimary-0468
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0468
Research groupRG Hasenfuß
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolPOU5F1
Antibody Registry ID(s)AB_354975
NameHuman/Mouse Oct-3/4 Antibody
Alternative nameMGC22487
Lab ID
Tag / Fluorophore
Raised inGoat
Reacts withHuman, Mouse
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenE. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu)
Crafted Bycompany
Company / ManufacturerR &D Systems
Catalog no.AF1759
Lot no.
DescriptionWestern Blot 0.5 µg/mL Simple Western 20 µg/mL Immunoprecipitation 3 µg/106 cells Chromatin Immunoprecipitation (ChIP) 5 µg/106 cells Immunocytochemistry 5-15 µg/mL
Localization
Storage instructionUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Receipt date2021-04-29 00:00:00
Preparation date2021-04-29 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes
Peper J, Kownatzki-Danger D, Weninger G, Seibertz F, Pronto JR, Sutanto H, Pacheu Grau D, Hindmarsh R, Brandenburg S, Kohl T, Hasenfuβ G, Gotthardt M, Rog-Zielinska EA, Wollnik B, Rehling P, Urlaub H, Wegener JW, Heijman J, Voigt N, Cyganek L, Lenz C, Lehnart SE
Circ Res 2021 : 10.1161/circresaha.119.316547

Oct4 primarily controls enhancer activity rather than accessibility
Xiong L, Tolen EA, Choi J, Caizzi L, Adachi K, Lidschreiber M, Cramer P, Schöler HR
bioRxiv 2021 : 10.1101/2021.06.28.450119

Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schöler HR
eLife 2022 : 10.7554/elife.71533

An Alternative Mechanism of Subcellular Iron Uptake Deficiency in Cardiomyocytes
Dai Y, Ignatyeva N, Xu H, Wali R, Toischer K, Brandenburg S, Lenz C, Pronto J, Fakuade FE, Sossalla S, Zeisberg EM, Janshoff A, Kutschka I, Voigt N, Urlaub H, Rasmussen TB, Mogensen J, Lehnart SE, Hasenfuss G, Ebert A
Circ Res 2023 : 10.1161/circresaha.122.321157