Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0468 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0468 |
| Research group | RG Hasenfuß |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | POU5F1 |
| Antibody Registry ID(s) | AB_354975 |
| Name | Human/Mouse Oct-3/4 Antibody |
| Alternative name | MGC22487 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Goat |
| Reacts with | human, mouse |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) |
| Crafted By | company |
| Company / Manufacturer | R &D Systems |
| Catalog no. | AF1759 |
| Lot no. | |
| Description | Western Blot 0.5 µg/mL Simple Western 20 µg/mL Immunoprecipitation 3 µg/106 cells Chromatin Immunoprecipitation (ChIP) 5 µg/106 cells Immunocytochemistry 5-15 µg/mL |
| Localization | |
| Storage instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Receipt date | 2021-04-29 00:00:00 |
| Preparation date | 2021-04-29 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
Applications
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Linked publications
Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes
Peper J, Kownatzki-Danger D, Weninger G, Seibertz F, Pronto JR, Sutanto H, Pacheu Grau D, Hindmarsh R, Brandenburg S, Kohl T, Hasenfuβ G, Gotthardt M, Rog-Zielinska EA, Wollnik B, Rehling P, Urlaub H, Wegener JW, Heijman J, Voigt N, Cyganek L, Lenz C, Lehnart SE
Circ Res 2021 : 10.1161/circresaha.119.316547
Oct4 primarily controls enhancer activity rather than accessibility
Xiong L, Tolen EA, Choi J, Caizzi L, Adachi K, Lidschreiber M, Cramer P, Schöler HR
bioRxiv 2021 : 10.1101/2021.06.28.450119
Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schöler HR
eLife 2022 : 10.7554/elife.71533
An Alternative Mechanism of Subcellular Iron Uptake Deficiency in Cardiomyocytes
Dai Y, Ignatyeva N, Xu H, Wali R, Toischer K, Brandenburg S, Lenz C, Pronto J, Fakuade FE, Sossalla S, Zeisberg EM, Janshoff A, Kutschka I, Voigt N, Urlaub H, Rasmussen TB, Mogensen J, Lehnart SE, Hasenfuss G, Ebert A
Circ Res 2023 : 10.1161/circresaha.122.321157
Generation of a pluripotent stem cell line (UMGi270-A) and a corresponding CRISPR/Cas9 modified isogenic control (UMGi270-A-1) from a patient with sudden onset dilated cardiomyopathy harboring a FLNC p.R2187P mutation
Maurer W, Rebs S, Köhne S, Eberl H, Wollnik B, Zibat A, Streckfuss-Bömeke K
Stem Cell Res 2024 : 10.1016/j.scr.2024.103409
