Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0344 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0344 |
| Research group | RG Preobraschenski |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | GFP |
| Antibody Registry ID(s) | AB_627695 |
| Name | Anti-GFP Antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | |
| Clone | B-2 |
| Isotype | IgG2a |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | raised against amino acids 1-238 representing full length GFP |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-9996 |
| Lot no. | |
| Description | GFP (B -2) is recommended for detection of GFP and GFP mutant fusion proteins by Western Blotting (starting dilution1:200, dilution range 1:100-1:1000), immunoprecipitation [1- 2 μg per 100-500 μg of total protein (1 ml of celllysate )], immunofluorescence (starting dilution 1:50, dilution range1:50-1:500 ), flowcytometry (1 μg per 1 x 106cel ls) and solid phase ELISA(starting dilution1:30, dilution range 1:30- 1:3000). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**.Stable for one year from the date of shipment. Non- hazardous. No MSDS required. |
| Receipt date | 2021-01-01 00:00:00 |
| Preparation date | 2021-04-17 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFP |
| Wikipedia | http://en.wikipedia.org/wiki/GFP |
| Uniprot | http://www.uniprot.org/uniprot/?query=GFP |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFP |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_627695 |
Applications
No application comments yet.
Linked publications
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2
Kreutzberger AJ, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anatharam A, Castle JD, Tamm LK
eLife 2020 : 10.7554/elife.62506
