View primary antibody

Basic data

PIDprimary-0344
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0344
Research groupRG Preobraschenski
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolGFP
Antibody Registry ID(s)AB_627695
NameAnti-GFP Antibody
Alternative name
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts with
CloneB-2
IsotypeIgG2a
Clonalitymonoclonal
Demaskingunknown
Antigenraised against amino acids 1-238 representing full length GFP
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-9996
Lot no.
DescriptionGFP (B -2) is recommended for detection of GFP and GFP mutant fusion proteins by Western Blotting (starting dilution1:200, dilution range 1:100-1:1000), immunoprecipitation [1- 2 μg per 100-500 μg of total protein (1 ml of celllysate )], immunofluorescence (starting dilution 1:50, dilution range1:50-1:500 ), flowcytometry (1 μg per 1 x 106cel ls) and solid phase ELISA(starting dilution1:30, dilution range 1:30- 1:3000).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**.Stable for one year from the date of shipment. Non- hazardous. No MSDS required.
Receipt date2021-01-01 00:00:00
Preparation date2021-04-17 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2
Kreutzberger AJ, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anatharam A, Castle JD, Tamm LK
eLife 2020 : 10.7554/elife.62506

Autophagy preferentially degrades non-fibrillar polyQ aggregates
Zhao DY, Bäuerlein FJ, Saha I, Hartl FU, Baumeister W, Wilfling F
Mol Cell 2024 : 10.1016/j.molcel.2024.04.018