View primary antibody

Basic data

PIDprimary-0255
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0255
Research groupRG Enderlein
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBA4A
Antibody Registry ID(s)AB_2210057
NameAnti-alpha Tubulin antibody
Alternative nameAlpha-tubulin 1 antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withMouse, Rat, Human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab18251
Lot no.
DescriptionICC/IF Use a concentration of 1 - 5 µg/ml. Flow Cyt Use 0.01µg for 106 cells. WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Abcam recommends using milk as the blocking agent.
LocalizationCytoplasm > cytoskeleton.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-03-31 00:00:00
Preparation date2021-03-31 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
Thiele JC, Helmerich DA, Oleksiievets N, Tsukanov R, Butkevich E, Sauer M, Nevskyi O, Enderlein J
ACS Nano 2020 : 10.1021/acsnano.0c07322

Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
bioRxiv 2021 : 10.1101/2021.12.20.473473

Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
Sci Adv 2022 : 10.1126/sciadv.abo2506