View primary antibody

Basic data

PIDprimary-0255
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0255
Research groupRG Enderlein
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBA4A
Antibody Registry ID(s)AB_2210057
NameAnti-alpha Tubulin antibody
Alternative nameAlpha-tubulin 1 antibody
Lab ID
Tag / Fluorophore
Raised inRabbit
Reacts withmouse, rat, human
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab18251
Lot no.
DescriptionICC/IF Use a concentration of 1 - 5 µg/ml. Flow Cyt Use 0.01µg for 106 cells. WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Abcam recommends using milk as the blocking agent.
LocalizationCytoplasm > cytoskeleton.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-03-31 00:00:00
Preparation date2021-03-31 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
Thiele JC, Helmerich DA, Oleksiievets N, Tsukanov R, Butkevich E, Sauer M, Nevskyi O, Enderlein J
ACS Nano 2020 : 10.1021/acsnano.0c07322

Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
bioRxiv 2021 : 10.1101/2021.12.20.473473

Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann M, Sauer M, Nevskyi O, Enderlein J
Sci Adv 2022 : 10.1126/sciadv.abo2506

Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm
Bredfeldt J, Oracz J, Kiszka KA, Moosmayer T, Weber M, Sahl SJ, Hell SW
Nat Commun 2024 : 10.1038/s41467-024-51160-9

One-step nanoscale expansion microscopy reveals individual protein shapes
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO
Nat Biotechnol 2024 : 10.1038/s41587-024-02431-9