View primary antibody

Basic data

PIDprimary-0226
EPIC PIDhttps://hdl.handle.net/21.11124/primary-0226
Research groupRG Cramer
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBA4A
Antibody Registry ID(s)AB_2241126
NameAnti-alpha Tubulin antibody
Alternative nameALS22 antibody
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human
CloneDM1A
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenFull length native protein (purified) corresponding to Chicken alpha Tubulin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab7291
Lot no.
Descriptionsfb1565: primary-0011 Flow Cyt Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. ICC/IF Use a concentration of 0.5 - 1 µg/ml. IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking
LocalizationCytoplasm > cytoskeleton.
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date2021-01-01 00:00:00
Preparation date2021-03-23 00:00:00
Created byKlauenberg, Vanessa
Last modified,

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Linked publications


CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response
Stik G, Vidal E, Barrero M, Cuartero S, Vila-Casadesús M, Mendieta-Esteban J, Tian TV, Choi J, Berenguer C, Abad A, Borsari B, le Dily F, Cramer P, Marti-Renom MA, Stadhouders R, Graf T
Nat Genet 2020 : 10.1038/s41588-020-0643-0