Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0226 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0226 |
| Research group | RG Cramer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | TUBA4A |
| Antibody Registry ID(s) | AB_2241126 |
| Name | Anti-alpha Tubulin antibody |
| Alternative name | ALS22 antibody |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | mouse, rat, human |
| Clone | DM1A |
| Isotype | IgG1 |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Full length native protein (purified) corresponding to Chicken alpha Tubulin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab7291 |
| Lot no. | |
| Description | sfb1565: primary-0011 Flow Cyt Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. ICC/IF Use a concentration of 0.5 - 1 µg/ml. IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking |
| Localization | Cytoplasm > cytoskeleton. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-01-01 00:00:00 |
| Preparation date | 2021-03-23 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=TUBA4A |
| Wikipedia | http://en.wikipedia.org/wiki/TUBA4A |
| Uniprot | http://www.uniprot.org/uniprot/?query=TUBA4A |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=TUBA4A |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2241126 |
Applications
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Linked publications
CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response
Stik G, Vidal E, Barrero M, Cuartero S, Vila-Casadesús M, Mendieta-Esteban J, Tian TV, Choi J, Berenguer C, Abad A, Borsari B, le Dily F, Cramer P, Marti-Renom MA, Stadhouders R, Graf T
Nat Genet 2020 : 10.1038/s41588-020-0643-0
