Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0053 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0053 |
| Research group | RG Fischer |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | H3K4me3 |
| Antibody Registry ID(s) | AB_306649 |
| Name | Anti-Histone H3 (tri methyl K4) antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Cow, Human |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4) conjugated to keyhole limpet haemocyanin. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab8580 |
| Lot no. | |
| Description | PepArr: Use a concentration of 0.2 - 0.02 µg/ml. Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimisation is recommended to avoid array signal saturation. ChIP: Use 2 µg for 25 µg of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as positive control. WB: Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). IHC-P: Use at an assay dependent concentration. ICC/IF: Use a concentration of 1 µg/ml. 1/100 - 1/5000 |
| Localization | Nucleus. Chromosome. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date | 2021-01-01 00:00:00 |
| Preparation date | 2021-01-20 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=H3K4me3 |
| Wikipedia | http://en.wikipedia.org/wiki/H3K4me3 |
| Uniprot | http://www.uniprot.org/uniprot/?query=H3K4me3 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=H3K4me3 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_306649 |
Applications
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Linked publications
Epigenetic gene-expression links heart failure to memory impairment
Islam R, Lbik D, Sakib S, Hofmann RM, Berulava T, Mausbach MJ, Cha J, Vakhtang E, Schiffmann C, Zieseniss A, Katschinski DM, Sananbenesi F, Toischer K, Fischer A
bioRxiv 2020 : 10.1101/2020.01.22.915637
Epigenetic gene expression links heart failure to memory impairment
Islam MR, Lbik D, Sakib MS, Maximilian Hofmann R, Berulava T, Jiménez Mausbach M, Cha J, Goldberg M, Vakhtang E, Schiffmann C, Zieseniss A, Katschinski DM, Sananbenesi F, Toischer K, Fischer A
EMBO Mol Med 2021 : 10.15252/emmm.201911900
SETD1B controls cognitive function via cell type specific regulation of neuronal identity genes
Michurina A, Sakib S, Kerimoglu C, Krüger DM, Kaurani L, Islam R, Centeno TP, Cha J, Xu X, Zeisberg EM, Kranz A, Stewart FA, Fischer A
bioRxiv 2020 : 10.1101/2020.08.07.240853
Postnatal expression of the lysine methyltransferase SETD1B is essential for learning and the regulation of neuron-enriched genes
Michurina A, Sakib MS, Kerimoglu C, Krüger DM, Kaurani L, Islam MR, Joshi PD, Schröder S, Centeno TP, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg EM, Kranz A, Stewart AF, Fischer A
EMBO J 2021 : 10.15252/embj.2020106459
Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3
Baidoe-Ansah D, Sakib S, Jia S, Mirzapourdelavar H, Strackeljan L, Fischer A, Aleshin S, Kaushik R, Dityatev A
Cells 2022 : 10.3390/cells11132033
