| Antigen symbol |
H3K4me3 |
| Antibody Registry ID(s) |
AB_306649 |
| Name |
Anti-Histone H3 (tri methyl K4) antibody |
| Alternative name |
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| Lab ID |
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| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
cow, human |
| Clone |
|
| Isotype |
IgG |
| Clonality |
polyclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4) conjugated to keyhole limpet haemocyanin. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab8580 |
| Lot no. |
|
| Description |
PepArr: Use a concentration of 0.2 - 0.02 µg/ml.
Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimisation is recommended to avoid array signal saturation.
ChIP: Use 2 µg for 25 µg of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as positive control.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 1 µg/ml. 1/100 - 1/5000 |
| Localization |
Nucleus. Chromosome. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Receipt date |
2021-01-01 00:00:00 |
| Preparation date |
2021-01-20 00:00:00 |
| Created by |
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| Last modified |
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