[{"version":"2","@context":"http:\/\/schema.org\/","@type":"Thing","additionalType":"Antibody","id":"53","pid":"primary-0053","epic_pid":"https:\/\/hdl.handle.net\/21.11124\/primary-0053","research_group":"RG Fischer","lab_id":"","type":"primary","antigen_symbol":"H3K4me3","antibody_registry_id":"AB_306649","name":"Anti-Histone H3 (tri methyl K4) antibody","alternative_name":"","tag_flurophore":"","raised_id":"Rabbit","reacts_with":"cow, human","clone":"","isotype":"IgG","clonality":"polyclonal","demasking":"unknown","antigen":"Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4) conjugated to keyhole limpet haemocyanin.","company":"Abcam","catalog_no":"ab8580","lot_no":"","description":"PepArr: Use a concentration of 0.2 - 0.02 \u00b5g\/ml.\r\nSlight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimisation is recommended to avoid array signal saturation.\r\nChIP: Use 2 \u00b5g for 25 \u00b5g of chromatin. We recommend GAPDH positive control ChIP primer pair ab267832 as positive control.\r\nWB: Use a concentration of 1 \u00b5g\/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).\r\nIHC-P: Use at an assay dependent concentration.\r\nICC\/IF: Use a concentration of 1 \u00b5g\/ml. 1\/100 - 1\/5000","last_modified":"Klauenberg, Vanessa"}]