| Antigen symbol |
RBFOX3 |
| Antibody Registry ID(s) |
AB_2532109 |
| Name |
Recombinant Anti-NeuN antibody [EPR12763] - Neuronal Marker |
| Alternative name |
FOX-3 |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
mouse, rat, sheep, goat, cat, dog, human, zebrafish, commonMarmoset |
| Clone |
EPR12763 |
| Isotype |
IgG |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Synthetic peptide. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab177487 |
| Lot no. |
|
| Description |
Flow Cyt (Intra)
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC (PFA fixed)
Use at an assay dependent concentration.
mIHC
1/1000.
Perform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.
IHC-P
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols.
For unpurified use at 1/800.
WB
1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).
For unpurified use at 1/1000 - 1/2000.
ICC/IF
1/100 - 1/300.
For unpurified use at 1/80.
IHC-Fr
Use at an assay dependent concentration. |
| Localization |
Nucleus. Cytoplasm. |
| Storage instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Receipt date |
2021-11-20 00:00:00 |
| Preparation date |
2021-11-20 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |