View primary antibody

Basic data

PID primary-0630
EPIC PID
Research group RG Wollnik
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol TUBB3
Antibody Registry ID(s) AB_444319
Name Anti-beta III Tubulin antibody
Alternative name CFEOM3
Lab ID
Tag / Fluorophore
Raised in
Reacts with mouse, rat, human, marmoset, dogfish, catshark
Clone
Isotype IgG
Clonality polyclonal
Demasking unknown
Antigen Synthetic peptide.
Crafted By company
Company / Manufacturer Abcam
Catalog no. ab18207
Lot no.
Description Flow Cyt Use 0.01µg for 106 cells. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ICC/IF Use a concentration of 1 - 5 µg/ml. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody WB Use a concentration of 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta III Tubulin peptide (ab18660).
Localization Cytoplasm > cytoskeleton.
Storage instruction Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date 2021-07-29 00:00:00
Preparation date 2021-07-29 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y et al.
Nat Commun 2021: 10.1038/s41467-021-23327-1