View primary antibody

Basic data

PID primary-0625
EPIC PID
Research group RG Wollnik
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol PAX3
Antibody Registry ID(s) AB_2722285
Name PAX3 Antibody
Alternative name HUP2
Lab ID
Tag / Fluorophore
Raised in
Reacts with human
Clone
Isotype MIgG1
Clonality monoclonal
Demasking unknown
Antigen Recombinant protein
Crafted By company
Company / Manufacturer DSHB
Catalog no. PCRP-PAX3-1B3
Lot no.
Description Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Localization
Storage instruction Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Receipt date 2021-07-29 00:00:00
Preparation date 2021-07-29 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


Overarching control of autophagy and DNA damage response by CHD6 revealed by modeling a rare human pathology
Kargapolova Y et al.
Nat Commun 2021: 10.1038/s41467-021-23327-1