View primary antibody

Basic data

PID primary-0545
EPIC PID
Research group RG Jakobs
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol ATP5F1B
Antibody Registry ID(s) AB_304883
Name Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker
Alternative name ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
Lab ID
Tag / Fluorophore
Raised in
Reacts with mouse, rat, human
Clone 4.3E8.D10
Isotype IgG1
Clonality monoclonal
Demasking unknown
Antigen Other Immunogen Type corresponding to ATPB. Intact rat mitochondria.
Crafted By company
Company / Manufacturer Abcam
Catalog no. ab5432
Lot no. GR3177231-2
Description WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). ICC/IF 1/100 - 1/1000. IP Use a concentration of 2 - 5 µg/ml. By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.
Localization Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
Storage instruction Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date 2021-05-20 00:00:00
Preparation date 2021-05-20 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


Colocalization for super-resolution microscopy via optimal transport
Tameling C et al.
Nat Comput Sci 2021: 10.1038/s43588-021-00050-x
 
DNA-PAINT MINFLUX nanoscopy
Ostersehlt LM et al.
Nat Methods 2022: 10.1038/s41592-022-01577-1
 
Super-resolution microscopy of mitochondrial mRNAs
Stoldt S et al.
bioRxiv 2025: 10.1101/2025.01.23.634455