| Antigen symbol |
SQSTM1 |
| Antibody Registry ID(s) |
AB_945626 |
| Name |
Anti-SQSTM1 / p62 antibody |
| Alternative name |
A170 antibody |
| Lab ID |
|
| Tag / Fluorophore |
|
| Raised in |
|
| Reacts with |
human |
| Clone |
2C11 |
| Isotype |
IgG2a |
| Clonality |
monoclonal |
| Demasking |
unknown |
| Antigen |
Recombinant full length protein corresponding to Human SQSTM1/ p62 aa 1-440. |
| Crafted By |
company |
| Company / Manufacturer |
Abcam |
| Catalog no. |
ab56416 |
| Lot no. |
|
| Description |
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Fix cells for 15 minutes with 2 mL of 4% paraformaldehyde solution (pH 7.4 with NaOH in PBS). Permeabilize cells by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS.
Flow Cyt
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
| Localization |
Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. |
| Storage instruction |
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Receipt date |
2021-04-20 00:00:00 |
| Preparation date |
2021-04-20 00:00:00 |
| Created by |
Klauenberg, Vanessa |
| Last modified |
Klauenberg, Vanessa |