View primary antibody

Basic data

PID primary-0178
EPIC PID
Research group RG Moser
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol NUP107
Antibody Registry ID(s) AB_448181
Name Anti-Nuclear Pore Complex Proteins antibody
Alternative name NPC proteins antibody
Lab ID
Tag / Fluorophore
Raised in
Reacts with mouse, human, SaccharomycesCerevisiae
Clone Mab414
Isotype IgG1
Clonality monoclonal
Demasking unknown
Antigen Nuclear pore complex mixture.
Crafted By company
Company / Manufacturer Abcam
Catalog no. ab24609
Lot no.
Description ICC/IF Use at an assay dependent concentration. Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this
Localization Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
Storage instruction Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date 2021-03-09 00:00:00
Preparation date 2021-03-09 00:00:00
Created by Klauenberg, Vanessa
Last modified Klauenberg, Vanessa

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Linked publications


Circumvention of common labeling artifacts using secondary nanobodies
Sograte-Idrissi S et al.
bioRxiv 2019: 10.1101/818351
 
Circumvention of common labelling artefacts using secondary nanobodies
Sograte-Idrissi S et al.
Nanoscale 2020: 10.1039/d0nr00227e