Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-1065 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-1065 |
| Research group | RG Rehling |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | FLAG |
| Antibody Registry ID(s) | AB_439687 |
| Name | ANTI-FLAG® antibody |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | all |
| Clone | |
| Isotype | IgG |
| Clonality | polyclonal |
| Demasking | unknown |
| Antigen | FLAG; peptide sequence DYKDDDDK |
| Crafted By | company |
| Company / Manufacturer | Sigma-Aldrich |
| Catalog no. | F7425 |
| Lot no. | |
| Description | dot blot: 1-2.5 μg/mL immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein |
| Localization | |
| Storage instruction | storage temp. −20°C |
| Receipt date | 2022-01-01 00:00:00 |
| Preparation date | 2022-08-14 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=FLAG |
| Wikipedia | http://en.wikipedia.org/wiki/FLAG |
| Uniprot | http://www.uniprot.org/uniprot/?query=FLAG |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=FLAG |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_439687 |
Applications
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Linked publications
HAX1-dependent control of mitochondrial proteostasis governs neutrophil granulocyte differentiation
Fan Y, Murgia M, Linder MI, Mizoguchi Y, Wang C, Łyszkiewicz M, Ziȩtara N, Liu Y, Frenz S, Sciuccati G, Partida-Gaytan A, Alizadeh Z, Rezaei N, Rehling P, Dennerlein S, Mann M, Klein C
J Clin Invest 2022 : 10.1172/jci153153
The SARS-CoV-2 protein ORF3c is a mitochondrial modulator of innate immunity
Stewart H, Lu Y, O’Keefe S, Valpadashi A, Cruz-Zaragoza LD, Michel HA, Nguyen SK, Carnell GW, Lukhovitskaya N, Milligan R, Adewusi Y, Jungreis I, Lulla V, Matthews DA, High S, Rehling P, Emmott E, Heeney JL, Davidson AD, Edgar JR, Smith GL, Firth AE
iScience 2023 : 10.1016/j.isci.2023.108080
