View primary antibody

Basic data

PIDprimary-1045
EPIC PIDhttps://hdl.handle.net/21.11124/primary-1045
Research groupRG Sakata
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolCDC27
Antibody Registry ID(s)AB_627228
NameAnti-Cdc27 Antibody (AF3.1)
Alternative nameAPC3
Lab ID
Tag / Fluorophore
Raised inMouse
Reacts withMouse, Rat, Human, Xenopus
CloneAF3.1
IsotypeIgG2b
Clonalitymonoclonal
Demaskingunknown
Antigenraised against amino acids 814-823 of Cdc27 of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-9972
Lot no.
DescriptionWestern Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total pro- tein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required
Receipt date2022-07-31 00:00:00
Preparation date2022-07-31 00:00:00
Created byKlauenberg, Vanessa
Last modifiedKlauenberg, Vanessa

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Linked publications


Allosteric control of Ubp6 and the proteasome via a bidirectional switch
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng X, Lu Y, Ovaa H, Engen JR, Lee B, Rudack T, Sakata E, Finley D
bioRxiv 2021 : 10.1101/2021.07.16.452743

Allosteric control of Ubp6 and the proteasome via a bidirectional switch
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng X, Lu Y, Ovaa H, Engen JR, Lee B, Rudack T, Sakata E, Finley D
Nat Commun 2022 : 10.1038/s41467-022-28186-y