Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-1045 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-1045 |
| Research group | RG Sakata |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | CDC27 |
| Antibody Registry ID(s) | AB_627228 |
| Name | Anti-Cdc27 Antibody (AF3.1) |
| Alternative name | APC3 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | mouse, rat, human, Xenopus |
| Clone | AF3.1 |
| Isotype | IgG2b |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | raised against amino acids 814-823 of Cdc27 of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-9972 |
| Lot no. | |
| Description | Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total pro- tein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required |
| Receipt date | 2022-07-31 00:00:00 |
| Preparation date | 2022-07-31 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
Applications
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Linked publications
Allosteric control of Ubp6 and the proteasome via a bidirectional switch
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng X, Lu Y, Ovaa H, Engen JR, Lee B, Rudack T, Sakata E, Finley D
bioRxiv 2021 : 10.1101/2021.07.16.452743
Allosteric control of Ubp6 and the proteasome via a bidirectional switch
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng X, Lu Y, Ovaa H, Engen JR, Lee B, Rudack T, Sakata E, Finley D
Nat Commun 2022 : 10.1038/s41467-022-28186-y
