Technical Support: menoci@medizin.uni-goettingen.de
Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0985 |
| EPIC PID | https://hdl.handle.net/21.11124/primary-0985 |
| Research group | RG Möbius |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | GAPDH |
| Antibody Registry ID(s) | AB_2630358 |
| Name | Recombinant Anti-GAPDH antibody [EPR16891] - Loading Control |
| Alternative name | |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Rabbit |
| Reacts with | Mouse, Rat, Chicken, Human, Zebrafish, AfricanGreenMonkey, XenopusTropicalis |
| Clone | EPR16891 |
| Isotype | IgG |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. |
| Crafted By | company |
| Company / Manufacturer | Abcam |
| Catalog no. | ab181602 |
| Lot no. | |
| Description | Flow Cyt (Intra) 1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. WB 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ICC/IF 1/500. IP 1/60. |
| Localization | Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. |
| Storage instruction | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Receipt date | 2022-03-16 00:00:00 |
| Preparation date | 2022-03-16 00:00:00 |
| Created by | Klauenberg, Vanessa |
| Last modified | Klauenberg, Vanessa |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=GAPDH |
| Wikipedia | http://en.wikipedia.org/wiki/GAPDH |
| Uniprot | http://www.uniprot.org/uniprot/?query=GAPDH |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=GAPDH |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2630358 |
Applications
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Linked publications
Mechanism of spindle pole organization and instability in human oocytes
So C, Menelaou K, Uraji J, Harasimov K, Steyer AM, Seres KB, Bucevičius J, Lukinavičius G, Möbius W, Sibold C, Tandler-Schneider A, Eckel H, Moltrecht R, Blayney M, Elder K, Schuh M
Science 2022 : 10.1126/science.abj3944
Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices
Jentoft IM, Bäuerlein FJ, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M
Cell 2023 : 10.1016/j.cell.2023.10.003
